Clone:
M1/70.15.11.5
Type of antibody:
Primary antibodies
Isotype:
rat IgG2bκ
Alternative names:
Integrin alpha-M, integrin αM, Mac-1 α, CR3A

Specifications for CD11b antibodies

Overview

M1/70.15.11.5 recognizes the human, mouse, and non-human primate CD11b antigen. CD11b, also known as Mac-1 α or integrin αM chain, is part of the CD11b/CD18 heterodimer (Mac-1 α, Mβ2 integrin), also known as the C3 complement receptor. It functions as a receptor for complement (C3bi), fibrinogen, or clotting factor X. In humans, CD11b is strongly expressed on myeloid cells and weakly expressed on NK cells and some activated lymphocytes as well as on microglia in the brain. In mice, the CD11b antigen is expressed on monocytes/macrophages and microglia. To a lower extent it is expressed on granulocytes, NK cells, CD5
+
B-1 cells, and subsets of dendritic cells.

Applications

Reagent can be used for immunophenotyping by flow cytometry. Abnormal numbers of cells expressing this antigen or aberrant expression levels of the antigen can be expected in some disease states. It is important to understand the normal expression pattern for this antigen and its relationship to expression of other relevant antigens in order to perform appropriate analysis.
Expression of CD11b may be used as aid to diagnostic in the characterization of samples from individuals suspected with hematologic neoplasia.

Alternative names

Integrin alpha-M, integrin αM, Mac-1 α, CR3A

Detailed product information

Technical specifications

CloneM1/70.15.11.5
Isotyperat IgG2bκ
Type of antibodyPrimary antibodies
Specieshuman
AntigenCD11b
Alternative names of antigenIntegrin alpha-M, integrin αM, Mac-1 α, CR3A
Molecular mass of antigen [kDa]125
Distribution of antigenB cells, dendritic cells, granulocytes, macrophages, monocytes, NK cells, T cells

Resources for CD11b antibodies

Certificates

Please follow this
link
to download the Certificate of Conformity (CoC) by lot number.

References for CD11b antibodies

Publications

  1. Stelzer GT et al. (1997) U.S.-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry 30(5): 214-230
  2. Lebson, L. et al. (2010) Trafficking CD11b-positive blood cells deliver therapeutic genes to the brain of amyloid-depositing transgenic mice. J. Neurosci. 30(29): 9651-9658
  3. Rothe, G et al. (2012) Consensus protocol for the flow cytometric Immunophenotyping of hematopoietic malignancies. Leukemia 10(5): 877-895
  4. van Dongen, J. J. M. et al. (2012) EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia 26(9): 1908-1975
  5. Liu, T. et al. (2014)
    Gr-1
    +
    CD11b
    +
    cells facilitate Lewis lung cancer recurrence by enhancing neovasculature after local irradiation.
    Sci Rep 4: 4833
  6. Clinical and Laboratory Standards Institute (CLSI) (2007) Clinical Flow Cytometric Analysis of Neoplastic Hematolympoid Cells, CLSI document H43-A2 (ISBN 1-56238-635-2) CLSI; Approved Guideline - Second Edition
  7. Clinical and Laboratory Standards Institute (CLSI) (2007) Enumeration of immunologically defined cell populations by flow cytometry, CLSI document H42-A2 (ISBN 1-56238-640-9) CLSI; Approved Guideline - Second Edition
  8. Wood BL et al. (2007) 2006 Bethesda International Consensus recommendations on the immunophenotypic analysis of hematolymphoid neoplasia by flow cytometry: optimal reagents and reporting for the flow cytometric diagnosis of hematopoietic neoplasia. Cytometry B Clin. Cytom. 72: 14-22

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