CD117 MicroBeads have been developed for the positive selection of hematopoietic stem and progenitor cells from mouse bone marrow . In addition, CD117 MicroBeads have been successfully used for the isolation of a variety of tissue stem cells, e.g. differentiated embryonic stem cell cultures
1
, or amniotic fluid-derived stem cells
2
.

Data and images for CD117 MicroBeads, mouse

Figures

Figure 1

Separation of bone marrow cells using CD117 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with lineage markers (biotinylated antibodies against CD5, CD11b, CD45R, Gr-1, 7-4, Ter-119 and Anti-Biotin-APC) and CD117-PE.
A:
B:
Before enrichment
Isolated CD117
+
cells after enrichment
View details

Figure 1

Separation of bone marrow cells using CD117 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with lineage markers (biotinylated antibodies against CD5, CD11b, CD45R, Gr-1, 7-4, Ter-119 and Anti-Biotin-APC) and CD117-PE.
View details

Figure 1

Separation of bone marrow cells using CD117 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with lineage markers (biotinylated antibodies against CD5, CD11b, CD45R, Gr-1, 7-4, Ter-119 and Anti-Biotin-APC) and CD117-PE.

Specifications for CD117 MicroBeads, mouse

Overview

CD117 MicroBeads have been developed for the positive selection of hematopoietic stem and progenitor cells from mouse bone marrow . In addition, CD117 MicroBeads have been successfully used for the isolation of a variety of tissue stem cells, e.g. differentiated embryonic stem cell cultures
1
, or amniotic fluid-derived stem cells
2
.

Detailed product information

Background information

CD117 (c-kit) is a receptor tyrosine kinase expressed on the majority of hematopoietic stem and progenitor cells, on some tissue stem cells as well as mature mast cells. Stimulation of CD117 by its ligand, the cytokine stem cell factor (SCF), is important for stem cell survival and maintance.

Downstream applications

  • Further flow cytometric analysis or sorting of isolated CD117+ cells, e.g. into LSK populations3.
  • Transplantation of CD117+ bone marrow cells, e.g. after myocardial infarction for differentiation into smooth muscle cells, myocytes, and endothelial cells4.

Columns

MS, LS, XS, or autoMACS
®
Columns.

Reviews for CD117 MicroBeads, mouse

Magnetic Sorting with Anti-Mouse CD117 Microbeads

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CD117 MicroBeads, mouse (130-091-224)

It has been shown that CD117+ is a marker of prostate basal stem cells. We used this anti-mouse CD117 magnetic microbeads to sort cells from the prostate, cells that constitute the putative population of basal cells that can reconstitute prostatic ducts. This research aimed to characterize the cell type(s) in the prostate that express a key marker of murine and human ESCs, and explore the hypothesis that aberrant expression of this marker may contribute to prostate inflammation/hyperplasia sometimes associated with prostatitis. We recommend this product.

References for CD117 MicroBeads, mouse

Publications

  1. Verda, L. et al. (2008) Hematopoietic mixed chimerism derived from allogeneic embryonic stem cells prevents autoimmune diabetes mellitus in NOD mice. Stem Cells 26: 381-386
  2. De Coppi, P. et al. (2007) Isolation of amniotic stem cell lines with potential for therapy. Nat. Biotechnol. 25: 100-106
  3. Shin, J.Y. et al. (2014) High c-Kit expression identifies hematopoietic stem cells with impaired self-renewal and megakaryocytic bias J. Exp. Med. 211(2): 217-231
  4. Orlic, D. et al. (2001) Mobilized bone marrow cells repair the infarcted heart, improving function and survival. Proc. Natl. Acad. Sci. U.S.A. 98: 10344-10349

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