Clone:
REA791
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
c-kit, SCO1, SCO5, SOW3, Ssm, Tr-kit, KIT, W, SCFR

Extended validation for CD117 Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA791
3C11++
2B8-
ACK2-
180627-
Cells were incubated with an excess of purified unconjugated CD117 (REA791) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD117. Bone marrow cells from C57BL/6 mice were stained with CD117 antibodies and with a suitable counterstaining. As a control, CD117 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD117. Bone marrow cells from C57BL/6 mice were stained with CD117 antibodies and with a suitable counterstaining. As a control, CD117 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD117. Bone marrow cells from C57BL/6 mice were stained with CD117 antibodies and with a suitable counterstaining. As a control, CD117 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD117. Bone marrow cells from C57BL/6 mice were stained with CD117 antibodies and with a suitable counterstaining. As a control, CD117 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD117. Bone marrow cells from C57BL/6 mice were stained with CD117 antibodies and with a suitable counterstaining. As a control, CD117 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD117. Bone marrow cells from C57BL/6 mice were stained with CD117 antibodies and with a suitable counterstaining. As a control, CD117 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD117 (REA791). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD117 (REA791). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD117 (REA791). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD117 Antibody, anti-mouse, REAfinity™

Overview

Clone REA791 recognizes the mouse CD117 antigen, a 145 kDa cell surface glycoprotein belonging to the class III receptor tyrosine kinase family. CD117 is also known as c-kit or stem cell factor (SCF) receptor. It is expressed on the majority of hematopoietic progenitor cells including multipotent hematopoietic stem cells as well as some committed myeloid, erythroid and lymphoid precursor cells, and mature mast cells. CD117
+
stem cells from murine bone marrow could also be differentiated into smooth muscle cells, myocytes, and endothelial cells
in vivo
.
Additional information: Clone REA791 displays negligible binding to Fc receptors.

Alternative names

c-kit, SCO1, SCO5, SOW3, Ssm, Tr-kit, KIT, W, SCFR

Detailed product information

Technical specifications

CloneREA791
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD117
Alternative names of antigenc-kit, SCO1, SCO5, SOW3, Ssm, Tr-kit, KIT, W, SCFR
Molecular mass of antigen [kDa]107
Distribution of antigenB cells, dendritic cells, endothelial cells, fibroblasts, megakaryocytes, T cells, NK cells, mast cells, cancer stem cells, hematopoietic stem and progenitor cells, bone marrow, brain, kidney, liver, lung
Entrez Gene ID16590
RRIDAB_2654591, AB_2654592, AB_2654593, AB_2654594, AB_2654595, AB_2654596, AB_2654597, AB_2654590

References for CD117 Antibody, anti-mouse, REAfinity™

Publications

  1. Qiu, F. H. et al. (1998) Primary structure of c-kit: relationship with the CSF-1/PDGF receptor kinase family--oncogenic activation of v-kit involves deletion of extracellular domain and C terminus. EMBO J. 7(4): 1003-1011
  2. Orlic, D. (2002) Stem cell repair in ischemic heart disease: an experimental model. Int. J. Hematol. 76(suppl.1): 144-145
  3. Zayas, J. et al. (2008) Murine hematopoietic stem cells and multipotent progenitors express truncated intracellular form of c-kit receptor. Stem Cells Dev. 17(2): 343-353

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