Clone:
AFS98
Type of antibody:
Primary antibodies
Isotype:
rat IgG2aκ
Applications:
FC, MICS, IF, IHC
Alternative names:
Csfmr, Fim-2, FMS, M-CSFR, CSF-1R, c-fms

Extended validation for CD115 Antibody, anti-mouse

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with AFS98
REA827+
T38-320-
460015-
REAL272-
Cells were incubated with an excess of purified unconjugated CD115 (AFS98) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD115. C57BL6/6 splenocytes were stained with CD115 antibodies and with a suitable counterstaining. As a control, CD115 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD115. C57BL6/6 splenocytes were stained with CD115 antibodies and with a suitable counterstaining. As a control, CD115 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD115. C57BL6/6 splenocytes were stained with CD115 antibodies and with a suitable counterstaining. As a control, CD115 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD115. C57BL6/6 splenocytes were stained with CD115 antibodies and with a suitable counterstaining. As a control, CD115 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD115. C57BL6/6 splenocytes were stained with CD115 antibodies and with a suitable counterstaining. As a control, CD115 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD115. C57BL6/6 splenocytes were stained with CD115 antibodies and with a suitable counterstaining. As a control, CD115 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD115 (AFS98). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD115 (AFS98). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD115 (AFS98). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD115 Antibody, anti-mouse

Overview

Clone AFS98 recognizes the protein tyrosine kinase transmembrane receptor for macrophage colony-stimulating factor (M-CSF), also known as CSF-1 and interleukin 34 (IL-34). CD115 is a single-pass type I membrane protein. Receptor activation induces homo-dimerization, which leads to phosphorylation and ubiquitinylation of intracellular residues. CD115 is expressed on mouse monocytes, macrophages, and osteoclasts, as well as on common dendritic cell precursors and macrophage/dendritic cell precursors.

Alternative names

Csfmr, Fim-2, FMS, M-CSFR, CSF-1R, c-fms

Detailed product information

Technical specifications

CloneAFS98
Clonalitymonoclonal
Isotyperat IgG2aκ
Isotype controlIsotype Control Antibody, rat IgG2a
Hostrat
Type of antibodyPrimary antibodies
Speciesmouse
AntigenCD115
Alternative names of antigenCsfmr, Fim-2, FMS, M-CSFR, CSF-1R, c-fms
Molecular mass of antigen [kDa]107
Distribution of antigendendritic cells, macrophages, osteoclasts, myeloid leukemia cells, breast, smooth muscle
Entrez Gene ID12978
RRIDAB_2660272, AB_2660273, AB_2660276, AB_2660277, AB_2660278, AB_2660279, AB_2660280, AB_2660281, AB_2819410

References for CD115 Antibody, anti-mouse

Publications

  1. Sunderkötter, C. et al. (2004) Subpopulations of mouse blood monocytes differ in maturation stage and inflammatory response. J. Immunol. 172: 4410-4417
  2. Auffray, C. et al. (2009)
    CX
    3
    CR1
    +
    CD115
    +
    CD135
    +
    common macrophage/DC precursors and the role of CX
    3
    CR1 in their response to inflammation.
    J. Exp. Med. 206(3): 595-606
  3. Murayama, T. et al. (1999) Intraperitoneal administration of anti-c-fms monoclonal antibody prevents initial events of atherogenesis but does not reduce the size of advanced lesions in apolipoprotein E-deficient mice. Circulation 99: 1740-1746
  4. Breslin W. L. et al. (2013) Mouse blood monocytes: Standardizing their identification and analysis using CD115. J. Immunol. Methods 390(1-2): 1-8
  5. Sudo, T. et al. (1995) Functional hierarchy of c-kit and c-fms in intramarrow production of CFU-M. Oncogene 11: 2469-2476

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