Clone:
REA1073
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC, IF, IHC
Alternative names:
LAMP-2, LAMPB, LGP110

Extended validation for CD107b Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA1073
H4B4++
508921+ +
Cells were incubated with an excess of purified unconjugated CD107b (REA1073) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD107b. Jurkat cells were fixed, permeabilized, and then stained with CD107b antibodies and plotted against the side scatter. As a control, CD107b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD107b. Jurkat cells were fixed, permeabilized, and then stained with CD107b antibodies and plotted against the side scatter. As a control, CD107b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD107b. Jurkat cells were fixed, permeabilized, and then stained with CD107b antibodies and plotted against the side scatter. As a control, CD107b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD107b. Jurkat cells were fixed, permeabilized, and then stained with CD107b antibodies and plotted against the side scatter. As a control, CD107b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD107b. Jurkat cells were fixed, permeabilized, and then stained with CD107b antibodies and plotted against the side scatter. As a control, CD107b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. The recommended titers of respective antibodies from different suppliers were used.

Specifications for CD107b Antibody, anti-human, REAfinity™

Overview

Clone REA1073 recognizes the human CD107b, also known as lysosomal-associated membrane protein-2 (LAMP-2), which is a 120 kDa highly glycosylated type I transmembrane protein. It is widely located in lysosomal/endosomal membranes in a high variety of cell types and also expressed on degranulating T cells, activated platelets, and some tumor cell lines. CD107b is involved in cell adhesion and cellular homeostasis, including autophagocytosis and antigen presentation as well as in tumor cell metastasis.
Additional information: Clone REA1073 displays negligible binding to Fc receptors.

Alternative names

LAMP-2, LAMPB, LGP110

Detailed product information

Technical specifications

CloneREA1073
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
AntigenCD107b
Alternative names of antigenLAMP-2, LAMPB, LGP110
Molecular mass of antigen [kDa]42
Distribution of antigenleukocytes, T cells, platelets, lymphocytes, tumor cells, tumor
Entrez Gene ID3920
RRIDAB_2751560, AB_2751572, AB_2751561, AB_2751573, AB_2751562, AB_2751574, AB_2751563, AB_2751575, AB_2751564, AB_2751576, AB_2751565, AB_2751577, AB_2751566, AB_2751578, AB_2751567, AB_2751571

References for CD107b Antibody, anti-human, REAfinity™

Publications

  1. Carlsson, S. R. et al. (1988) Isolation and characterization of human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Major sialoglycoproteins carrying polylactosaminoglycan. J. Biol. Chem. 263: 18911-18919
  2. Betts, M. R. et al. (2004) Detection of T-cell degranulation: CD107a and b. Method Cell Biol. 75: 497-512
  3. Grutzkau, A. et al. (2004) LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells. Cytometry A 61(1): 62-68

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