Clone:
2E7
Type of antibody:
Primary antibodies
Isotype:
hamster IgG
Applications:
FC, MICS, IF, IHC
Alternative names:
ITGAE, aM290, alpha-E1, alpha-M290, integrin alpha E

Extended validation for CD103 Antibody, anti-mouse

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with 2E7
REA789++
Cells were incubated with an excess of purified unconjugated CD103 (2E7) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD103. Splenocytes from C57BL/6 mice were stained with CD103 antibodies and with a suitable counterstaining. As a control, CD103 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD103. Splenocytes from C57BL/6 mice were stained with CD103 antibodies and with a suitable counterstaining. As a control, CD103 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD103. Splenocytes from C57BL/6 mice were stained with CD103 antibodies and with a suitable counterstaining. As a control, CD103 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD103. Splenocytes from C57BL/6 mice were stained with CD103 antibodies and with a suitable counterstaining. As a control, CD103 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD103. Splenocytes from C57BL/6 mice were stained with CD103 antibodies and with a suitable counterstaining. As a control, CD103 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD103. Splenocytes from C57BL/6 mice were stained with CD103 antibodies and with a suitable counterstaining. As a control, CD103 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for CD103 Antibody, anti-mouse

Overview

Armenian hamster IgG Clone 2E7 reacts with mouse CD103, a 175 kDa large, type I transmembrane glycoprotein also known as αE integrin. CD103 associates non-covalently with integrin β7 thereby forming the heterodimeric integrin αEβ7. It mediates cell-cell contact and is involved in homing processes by binding to its ligand e-cadherin. CD103 is expressed on >90% of intestinal intraepithelial lymphocytes (IEL), epithelial T cells, and regulatory T cells. Due to its differential expression on dendritic cells from peripheral tissues, it is useful in identifying dendritic cell subsets from intestinal lamina propria, lung, and dermis.

Alternative names

ITGAE, aM290, alpha-E1, alpha-M290, integrin alpha E

Detailed product information

Technical specifications

Clone2E7
Clonalitymonoclonal
Isotypehamster IgG
Hosthamster
Type of antibodyPrimary antibodies
Speciesmouse
AntigenCD103
Alternative names of antigenITGAE, aM290, alpha-E1, alpha-M290, integrin alpha E
Molecular mass of antigen [kDa]127
Distribution of antigenT cells, B cells, lymphocytes, thymocytes, leukemia cells, breast, pancreas, skin, small intestine, spleen
Entrez Gene ID16407
RRIDAB_2751541, AB_2783898, AB_2654399, AB_2654400, AB_2751551

References for CD103 Antibody, anti-mouse

Publications

  1. Sung, S. S. et al. (2006) A major lung CD103 alphaE-beta7 integrin-positive epithelial dendritic cell population expressing Langerin and tight junction proteins. J. Immunol. 176(9): 2161-2172
  2. Bogunovic, M. et al. (2009) Origin of the lamina propria dendritic cell network. Immunity 31(3): 513-525
  3. Ginhoux, F. et al. (2007)
    Blood-derived dermal Langerin
    +
    dendritic cells survey the skin in the steady state.
    J. Exp. Med. 204(13): 3133-3146