Clone:
REA326
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IHC, MC
Alternative names:
GPR2, G-protein coupled receptor 2 (GPR2, GPR-2)

Extended validation for CCR10 Antibody, anti-human, REAfinity™

Sensitivity

Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CCR10 (REA326). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CCR10 (REA326). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CCR10 (REA326). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CCR10 Antibody, anti-human, REAfinity™

Overview

Clone REA326 recognizes the human C-C chemokine receptor type 10 (CCR10) antigen, a multi-pass membrane protein which is also known as G-protein–coupled receptor 2 (GPR2). CCR10 is expressed by various subsets of innate-like T cells that are programmed to localize to the skin during their developmental processes in the thymus. Circulating T cells might be imprinted by skin-associated antigen-presenting cells to express CCR10 for their recruitment to the skin during the local immune response. On the other hand, IgA antibody–producing B cells generated in mucosa-associated lymphoid tissues express CCR10 for their migration and maintenance at mucosal sites. CCR10 and its ligands chemokines CCL27 and CCL28 are uniquely involved in the epithelial immunity. CCL27 is up-regulated in inflamed skin, whereas CCL28 is selectively expressed in intestinal epithelium.
In vitro
, CCL27−CCR10 interactions mediate the preferential migration of skin-homing CLA-bearing memory T cells. Increasing evidence also found that CCR10/ligands are involved in regulation of other immune cells in epithelial immunity and are frequently exploited by epithelium-localizing or -originated cancer cells for their survival, proliferation, and evasion from immune surveillance.
Additional information: Clone REA326 displays negligible binding to Fc receptors.

Alternative names

GPR2, G-protein coupled receptor 2 (GPR2, GPR-2)

Detailed product information

Technical specifications

CloneREA326
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCCR10
Alternative names of antigenGPR2, G-protein coupled receptor 2 (GPR2, GPR-2)
Molecular mass of antigen [kDa]38
Distribution of antigenB cells, T cells
Entrez Gene ID2826
RRIDAB_2752080, AB_2752131, AB_2752079, AB_2801898, AB_2752132

References for CCR10 Antibody, anti-human, REAfinity™

Publications

  1. Jarmin, D. I. et al. (2000) Cutting edge: identification of the orphan receptor G-protein-coupled receptor 2 as CCR10, a specific receptor for the chemokine ESkine. J. Immunol. 164(7): 3460-3464
  2. Sisirak, V. et al. (2011) CCR6/CCR10-mediated plasmacytoid dendritic cell recruitment to inflamed epithelia after instruction in lymphoid tissues. Blood 118(19): 5130-5140
  3. Brugman, S. et al. (2018) BAFF augments IgA2 and IL-10 production by TLR7/8 stimulated total peripheral blood B cells. Eur. J. Immunol. 48(24): 283-292
  4. Homey, B. et al. (2002) CCL27-CCR10 interactions regulate T cell-mediated skin inflammation. Nat Med 8(2): 157-165
  5. Fenton, K. A. et al. (2018) Mesenchymal stem cells and T cells in the formation of tertiary lymphoid structures in lupus nephritis. Sci Rep 8(1): 7861
  6. Xiong, N. et al. (2012) CCR10 and its ligands in regulation of epithelial immunity and diseases. Protein Cell 3(8): 571-580

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