The Cardiac Progenitor Cell Isolation Kit (Sca-1) has been specifically developed for the isolation of cardiac progenitor cells from mouse heart tissue.

Data and images for Cardiac Progenitor Cell Isolation Kit (Sca-1), mouse

Figures

Figure 1

Sca-1
+
/CD31
-
cardiac progenitor cells were isolated from dissociated mouse heart tissue by using the Cardiac Progenitor Isolation Kit (Sca-1), an LS and an MS Column, a MidiMACS™ and a MiniMACS™ Separator. The cells were fluorescently stained with Anti-Biotin-FITC (# 130-090-857) and Anti-Sca-1-APC (# 130-093-223) and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Unseparated fraction
CD31-depleted cells
View details

Figure 1

Sca-1
+
/CD31
-
cardiac progenitor cells were isolated from dissociated mouse heart tissue by using the Cardiac Progenitor Isolation Kit (Sca-1), an LS and an MS Column, a MidiMACS™ and a MiniMACS™ Separator. The cells were fluorescently stained with Anti-Biotin-FITC (# 130-090-857) and Anti-Sca-1-APC (# 130-093-223) and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Sca-1
+
/CD31
-
cardiac progenitor cells were isolated from dissociated mouse heart tissue by using the Cardiac Progenitor Isolation Kit (Sca-1), an LS and an MS Column, a MidiMACS™ and a MiniMACS™ Separator. The cells were fluorescently stained with Anti-Biotin-FITC (# 130-090-857) and Anti-Sca-1-APC (# 130-093-223) and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Sca-1
+
CD31
-
cardiac progenitor cells
View details

Figure 1

Sca-1
+
/CD31
-
cardiac progenitor cells were isolated from dissociated mouse heart tissue by using the Cardiac Progenitor Isolation Kit (Sca-1), an LS and an MS Column, a MidiMACS™ and a MiniMACS™ Separator. The cells were fluorescently stained with Anti-Biotin-FITC (# 130-090-857) and Anti-Sca-1-APC (# 130-093-223) and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Cardiac Progenitor Cell Isolation Kit (Sca-1), mouse

Overview

The Cardiac Progenitor Cell Isolation Kit (Sca-1) has been specifically developed for the isolation of cardiac progenitor cells from mouse heart tissue.

Detailed product information

Background information

A population of cardiac progenitor cells from the adult myocardium based on their expression of stem cell antigen-1 (Sca-1) has been identified.
1
These cells express most cardiogenic transcription factors but lack expression of cardiac structural genes. After treatment with 5-azacytidine, cardiac differentiation was induced in Sca-1
+
cells
in vitro
. In addition, this population homed to the injured myocardium upon grafting in an ischemia/reperfusion mouse model following by cardiac differentiation and fusion to the host myocardium. The majority of Sca-1
+
cells in the adult mouse heart co-express the endothelial marker CD31.
1,2
It has been shown that only the CD31
-
but not CD31
+
population exhibit functional cardiomyogenic differentiation.
2
In conclusion, Sca-1
+
/CD31
-
cells represent a distinct cardiac progenitor cell population, capable of differentiation into mature cardiomyocytes.

Columns

MS, LS, or autoMACS
®
Columns.

References for Cardiac Progenitor Cell Isolation Kit (Sca-1), mouse

Publications

  1. Oh, H. et al. (2003) Cardiac progenitor cells from adult myocardium: homing, differentiation, and fusion after infarction. Proc. Natl. Acad. Sci. U.S.A. 100: 12313-12318
  2. Pfister, O. et al. (2005)
    CD31
    but not CD31
    +
    cardiac side population cells exhibit functional cardiomyogenic differentiation.
    Circ. Res. 97: 52-61

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