Clone:
REAL864
Type of antibody:
Releasable fluorochromes, Primary antibodies, Recombinant antibodies
Applications:
MICS, IHC, IF
Alternative names:
BCL2

Extended validation for Bcl-2 Antibody, anti-human, REAdye_lease™

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for Bcl-2. Human peripheral blood mononuclear cells (PBMCs) were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Transcription Factor Staining Buffer Set followed by intracellular staining with Bcl-2 antibodies. As a control, Bcl-2antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Bcl-2. Human peripheral blood mononuclear cells (PBMCs) were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Transcription Factor Staining Buffer Set followed by intracellular staining with Bcl-2 antibodies. As a control, Bcl-2antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Bcl-2. Human peripheral blood mononuclear cells (PBMCs) were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Transcription Factor Staining Buffer Set followed by intracellular staining with Bcl-2 antibodies. As a control, Bcl-2antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Bcl-2. Human peripheral blood mononuclear cells (PBMCs) were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Transcription Factor Staining Buffer Set followed by intracellular staining with Bcl-2 antibodies. As a control, Bcl-2antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for Bcl-2. Human peripheral blood mononuclear cells (PBMCs) were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Transcription Factor Staining Buffer Set followed by intracellular staining with Bcl-2 antibodies. As a control, Bcl-2antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for Bcl-2 Antibody, anti-human, REAdye_lease™

Overview

Clone REAL864 is an antibody fragment derived from the full Bcl-2 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAdye_lease Complex to bind markers with high avidity.
Clone REAL864 recognizes the human Bcl-2 antigen, which is an intracellular single-pass membrane protein found primarily in the mitochondrion outer membrane, the nucleus membrane, and the endoplasmic reticulum membrane. Bcl-2 is a member of the Bcl-2 family of regulator proteins and forms homodimers or heterodimers with other Bcl-2 family members. It suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Bcl-2 regulates cell death by controlling the mitochondrial membrane permeability and it appears to function in a feedback loop system with caspases. Caspase activity is either inhibited by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor. BCL-2 antibody is suited for the differentiation between reactive and neoplastic follicular proliferation in lymph nodes and could be a useful marker for breast cancer and non-small cell carcinoma of the lung.
For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675).

Alternative names

BCL2

Detailed product information

Technical specifications

CloneREAL864
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable fluorochromes, Primary antibodies, Recombinant antibodies
Specieshuman
AntigenBcl-2
Alternative names of antigenBCL2
Distribution of antigenneurons, tonsil
RRIDAB_2904643

Resources for Bcl-2 Antibody, anti-human, REAdye_lease™

Certificates

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References for Bcl-2 Antibody, anti-human, REAdye_lease™

Publications

  1. Tsujimoto, Y. et al. (1986) Analysis of the structure, transcripts, and protein products of bcl-2, the gene involved in human follicular lymphoma. Proc. Natl. Acad. Sci. U.S.A. 83(14): 5214-5218
  2. Ciocca, D. R. et al. (2000) Molecular markers for predicting response to tamoxifen in breast cancer patients. Endocrine 13(1): 1-10
  3. Martin, B. et al. (2003) Role of Bcl-2 as a prognostic factor for survival in lung cancer: a systematic review of the literature with meta-analysis. Br. J. Cancer 89(1): 55-64

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