Anti-TRA-1-60 MicroBeads, human

Name: Anti-TRA-1-60 MicroBeads, human
Availability: InStock

Anti-TRA-1-60 MicroBeads, human

The direct Anti-TRA-1-60 MicroBeads and the indirect Anti-TRA-1-60 MicroBead Kit have been developed for the positive selection of human pluripotent stem cells based on the expression of the TRA-1-60 antigen.

The direct Anti-TRA-1-60 MicroBeads offer a 10 min isolation procedure, minimizing cell handling time.

Product data and images for Anti-TRA-1-60 MicroBeads, human

Figures

Figure 1

Pluripotent (TRA-1-60

⁺

) iPSCs were isolated from cultures containing spontaneously differentiated iPSCs grown on mouse embryonic feeder cells using Anti-TRA-1-60 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-TRA-1-60-PE (# 130-100-347) and Anti-Feeder-APC after separation and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Unseparated fraction

Enriched TRA-1-60

⁺

pluripotent stem cells

Figure 1

Pluripotent (TRA-1-60

⁺

) iPSCs were isolated from cultures containing spontaneously differentiated iPSCs grown on mouse embryonic feeder cells using Anti-TRA-1-60 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-TRA-1-60-PE (# 130-100-347) and Anti-Feeder-APC after separation and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 1

Pluripotent (TRA-1-60

⁺

) iPSCs were isolated from cultures containing spontaneously differentiated iPSCs grown on mouse embryonic feeder cells using Anti-TRA-1-60 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-TRA-1-60-PE (# 130-100-347) and Anti-Feeder-APC after separation and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Anti-TRA-1-60 MicroBeads, human

Overview

The direct Anti-TRA-1-60 MicroBeads and the indirect Anti-TRA-1-60 MicroBead Kit have been developed for the positive selection of human pluripotent stem cells based on the expression of the TRA-1-60 antigen.

The direct Anti-TRA-1-60 MicroBeads offer a 10 min isolation procedure, minimizing cell handling time.

Detailed product information

Background information

The Anti-TRA-1-60 monoclonal antibody reacts with a pluripotent stem cell–specific antigen expressed on undifferentiated human embryonic stem (ES) cells, induced pluripotent (iPS) cells, embryonal carcinoma (EC) cells, and embryonic germ (EG) cells.

^2,3

The expression of TRA-1-60 on human ES cells is down-regulated upon differentiation. The Anti-TRA-1-60 antibody recognizes a neuraminidase-resistant carbohydrate epitope expressed on podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein, which has been implicated in the development of aggressiveness in a variety of cancers including breast cancer and prostate cancer.

Applications

Positive selection of undifferentiated TRA-1-60⁺ pluripotent stem cells, for example, human ES and iPS cells.
Efficient enrichment of iPS cells during or after reprogramming^1,5.

Columns

MS or autoMACS

^®

Columns for use with Anti-TRA-1-60 MicroBeads.

LS or autoMACS Columns for use with the Anti-TRA-1-60 MicroBead Kit.

Resources for Anti-TRA-1-60 MicroBeads, human

Documents and Protocols

Brochures/posters

Pluripotent stem cell research

Scientific posters

Sequential enrichment of TRA-1-60+ PSC and CD271 (p75)+ NCSC enhances peripheral neuron differentiation

App notes/customer reports

Isolation of human induced pluripotent stem cell lines. Dick et al. (2012) MACS&more 14(2).

References for Anti-TRA-1-60 MicroBeads, human

Publications

Dick, E. et al. (2011) Faster generation of hiPSCs by coupling high-titer lentivirus and column-based positive selection. Nat. Protoc. 6(6): 701-714
Andrews, P. W. et al. (1984) Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells. Hybridoma 3: 347-361
Chan, E. M. et al. (2009) Live cell imaging distinguishes bona fide human iPS cells from partially reprogrammed cells. Nat. Biotechnol. 27: 1033-1037
Grabundzija, I. et al. (2013) Sleeping Beauty transposon-based system for cellular reprogramming and targeted gene insertion in induced pluripotent stem cells. Nucleic Acids Res. 41(3): 1829-1847
Tanabe, K. et al. (2013) Maturation, not initiation, is the major roadblock during reprogramming toward pluripotency from human fibroblasts. Proc. Natl. Acad. Sci. U.S.A. 110: 12172-12179

Data and images

Data and images for Anti-TRA-1-60 MicroBeads, human

Figures

Figure 1

Pluripotent (TRA-1-60

⁺

) iPSCs were isolated from cultures containing spontaneously differentiated iPSCs grown on mouse embryonic feeder cells using Anti-TRA-1-60 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-TRA-1-60-PE (# 130-100-347) and Anti-Feeder-APC after separation and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Unseparated fraction

Enriched TRA-1-60

⁺

pluripotent stem cells

Figure 1

Pluripotent (TRA-1-60

⁺

) iPSCs were isolated from cultures containing spontaneously differentiated iPSCs grown on mouse embryonic feeder cells using Anti-TRA-1-60 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-TRA-1-60-PE (# 130-100-347) and Anti-Feeder-APC after separation and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 1

Pluripotent (TRA-1-60

⁺

) iPSCs were isolated from cultures containing spontaneously differentiated iPSCs grown on mouse embryonic feeder cells using Anti-TRA-1-60 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-TRA-1-60-PE (# 130-100-347) and Anti-Feeder-APC after separation and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications

Specifications for Anti-TRA-1-60 MicroBeads, human

Overview

The direct Anti-TRA-1-60 MicroBeads and the indirect Anti-TRA-1-60 MicroBead Kit have been developed for the positive selection of human pluripotent stem cells based on the expression of the TRA-1-60 antigen.

The direct Anti-TRA-1-60 MicroBeads offer a 10 min isolation procedure, minimizing cell handling time.

Detailed product information

Background information

The Anti-TRA-1-60 monoclonal antibody reacts with a pluripotent stem cell–specific antigen expressed on undifferentiated human embryonic stem (ES) cells, induced pluripotent (iPS) cells, embryonal carcinoma (EC) cells, and embryonic germ (EG) cells.

^2,3

The expression of TRA-1-60 on human ES cells is down-regulated upon differentiation. The Anti-TRA-1-60 antibody recognizes a neuraminidase-resistant carbohydrate epitope expressed on podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein, which has been implicated in the development of aggressiveness in a variety of cancers including breast cancer and prostate cancer.

Applications

Positive selection of undifferentiated TRA-1-60⁺ pluripotent stem cells, for example, human ES and iPS cells.
Efficient enrichment of iPS cells during or after reprogramming^1,5.

Columns

MS or autoMACS

^®

Columns for use with Anti-TRA-1-60 MicroBeads.

LS or autoMACS Columns for use with the Anti-TRA-1-60 MicroBead Kit.

Resources

Resources for Anti-TRA-1-60 MicroBeads, human

Documents and Protocols

Brochures/posters

References for Anti-TRA-1-60 MicroBeads, human

Publications

Dick, E. et al. (2011) Faster generation of hiPSCs by coupling high-titer lentivirus and column-based positive selection. Nat. Protoc. 6(6): 701-714
Andrews, P. W. et al. (1984) Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells. Hybridoma 3: 347-361
Chan, E. M. et al. (2009) Live cell imaging distinguishes bona fide human iPS cells from partially reprogrammed cells. Nat. Biotechnol. 27: 1033-1037
Grabundzija, I. et al. (2013) Sleeping Beauty transposon-based system for cellular reprogramming and targeted gene insertion in induced pluripotent stem cells. Nucleic Acids Res. 41(3): 1829-1847
Tanabe, K. et al. (2013) Maturation, not initiation, is the major roadblock during reprogramming toward pluripotency from human fibroblasts. Proc. Natl. Acad. Sci. U.S.A. 110: 12172-12179

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Anti-TRA-1-60 MicroBeads, human

Anti-TRA-1-60 MicroBeads, human

Product data and images for Anti-TRA-1-60 MicroBeads, human

Figures

Figure 1