Anti SSEA-1 (CD15) MicroBeads are the first product to allow magnetic cell separation based on the expression of SSEA-1. In less than an hour, it is possible to
  • separate pluripotent mouse ES or iPS cells from heterogeneous cultures, e.g. to eliminate feeder cells,
  • enrich reprogrammed mouse iPS cells,
  • deplete unwanted pluripotent cells from ES or iPS cell-derived differentiation cultures,
  • deplete human ES or iPS cells that have undergone spontaneous differentiation.

Data and images for Anti-SSEA-1 (CD15) MicroBeads, human and mouse

Figures

Figure 1

Isolation of mouse ES cells using Anti-SSEA-1 (CD15) MicroBeads, an LS Column, and a MidiMACS™ Separator. Samples from a co-culture of mouse ES cells and embryonic fibroblasts expressing H-2K
k
were taken either before or after MACS
®
Separation and stained with anti-H-2K
k
-PE before analysis by flow cytometry. Expression of ectopic H-2K
k
was performed solely as a means of identifying fibroblasts within mixed cultures and is not required for magnetic separation.
Unseparated fraction
Negative fraction
View details

Figure 1

Isolation of mouse ES cells using Anti-SSEA-1 (CD15) MicroBeads, an LS Column, and a MidiMACS™ Separator. Samples from a co-culture of mouse ES cells and embryonic fibroblasts expressing H-2K
k
were taken either before or after MACS
®
Separation and stained with anti-H-2K
k
-PE before analysis by flow cytometry. Expression of ectopic H-2K
k
was performed solely as a means of identifying fibroblasts within mixed cultures and is not required for magnetic separation.
View details

Figure 1

Isolation of mouse ES cells using Anti-SSEA-1 (CD15) MicroBeads, an LS Column, and a MidiMACS™ Separator. Samples from a co-culture of mouse ES cells and embryonic fibroblasts expressing H-2K
k
were taken either before or after MACS
®
Separation and stained with anti-H-2K
k
-PE before analysis by flow cytometry. Expression of ectopic H-2K
k
was performed solely as a means of identifying fibroblasts within mixed cultures and is not required for magnetic separation.
Isolated mouse ES cells
View details

Figure 1

Isolation of mouse ES cells using Anti-SSEA-1 (CD15) MicroBeads, an LS Column, and a MidiMACS™ Separator. Samples from a co-culture of mouse ES cells and embryonic fibroblasts expressing H-2K
k
were taken either before or after MACS
®
Separation and stained with anti-H-2K
k
-PE before analysis by flow cytometry. Expression of ectopic H-2K
k
was performed solely as a means of identifying fibroblasts within mixed cultures and is not required for magnetic separation.

Specifications for Anti-SSEA-1 (CD15) MicroBeads, human and mouse

Overview

Anti SSEA-1 (CD15) MicroBeads are the first product to allow magnetic cell separation based on the expression of SSEA-1. In less than an hour, it is possible to
  • separate pluripotent mouse ES or iPS cells from heterogeneous cultures, e.g. to eliminate feeder cells,
  • enrich reprogrammed mouse iPS cells,
  • deplete unwanted pluripotent cells from ES or iPS cell-derived differentiation cultures,
  • deplete human ES or iPS cells that have undergone spontaneous differentiation.

Detailed product information

Background information

In the murine system, SSEA-1 is one of the key embryonic stem (ES) cell markers associated with the state of pluripotency. It is known to be expressed on undifferentiated murine ES and induced pluripotent stem (iPS) cells.
1-4
In contrast, SSEA-1 is not expressed on undifferentiated human ES cells but becomes upregulated during spontaneous differentiation.
5

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

References for Anti-SSEA-1 (CD15) MicroBeads, human and mouse

Publications

  1. Durcova et al. (1998) J. Reprod. Dev. 44: 85-89
  2. Cui, L. et al. (2004) Spatial distribution and initial changes of SSEA-1 and other cell adhesion-related molecules on mouse embryonic stem cells before and during differentiation. J. Histochem. Cytochem. 52: 1447-1457
  3. Shin, S. et al. (2007) Stage-dependent Olig2 expression in motor neurons and oligodendrocytes differentiated from embryonic stem cells. Stem Cells Dev. 16: 131-141
  4. Stadtfeld et al. (2008) Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse. Cell Stem Cell 6: 230-240
  5. International Stem Cell Initative (2007) Nat. Biotechnol. 25(7): 151-159

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