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Siglec-F + eosinophils were isolated from mouse peripheral blood using Anti-Siglec-F MicroBeads, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-Siglec-F-APC and Anti-Gr-1-VioGreen™ and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
Before separation | Siglec-F + cells |
Anti-Siglec-F MicroBeads, mouseFigure 1Siglec-F + eosinophils were isolated from mouse peripheral blood using Anti-Siglec-F MicroBeads, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-Siglec-F-APC and Anti-Gr-1-VioGreen™ and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. | Anti-Siglec-F MicroBeads, mouseFigure 1Siglec-F + eosinophils were isolated from mouse peripheral blood using Anti-Siglec-F MicroBeads, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-Siglec-F-APC and Anti-Gr-1-VioGreen™ and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
Siglec-F + alveolar macrophages and eosinophils were isolated from mouse lung single-cell suspension using Anti-Siglec-F MicroBeads, two MS Columns, and a MiniMACS Separator. Cells were fluorescently stained with Anti-Siglec-F-APC and CD11c-PE‑Vio ® 770 and analyzed by flow cytometry using the MACSQuant Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
Before separation | Siglec-F + cells |
Anti-Siglec-F MicroBeads, mouseFigure 2Siglec-F + alveolar macrophages and eosinophils were isolated from mouse lung single-cell suspension using Anti-Siglec-F MicroBeads, two MS Columns, and a MiniMACS Separator. Cells were fluorescently stained with Anti-Siglec-F-APC and CD11c-PE‑Vio ® 770 and analyzed by flow cytometry using the MACSQuant Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. | Anti-Siglec-F MicroBeads, mouseFigure 2Siglec-F + alveolar macrophages and eosinophils were isolated from mouse lung single-cell suspension using Anti-Siglec-F MicroBeads, two MS Columns, and a MiniMACS Separator. Cells were fluorescently stained with Anti-Siglec-F-APC and CD11c-PE‑Vio ® 770 and analyzed by flow cytometry using the MACSQuant Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
Siglec-F + eosinophils were isolated from mouse peripheral blood using Anti-Siglec-F MicroBeads, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-Siglec-F-APC and Anti-Gr-1-VioGreen™ and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
Before separation | Siglec-F + cells |
Anti-Siglec-F MicroBeads, mouseFigure 1Siglec-F + eosinophils were isolated from mouse peripheral blood using Anti-Siglec-F MicroBeads, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-Siglec-F-APC and Anti-Gr-1-VioGreen™ and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. | Anti-Siglec-F MicroBeads, mouseFigure 1Siglec-F + eosinophils were isolated from mouse peripheral blood using Anti-Siglec-F MicroBeads, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-Siglec-F-APC and Anti-Gr-1-VioGreen™ and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
Siglec-F + alveolar macrophages and eosinophils were isolated from mouse lung single-cell suspension using Anti-Siglec-F MicroBeads, two MS Columns, and a MiniMACS Separator. Cells were fluorescently stained with Anti-Siglec-F-APC and CD11c-PE‑Vio ® 770 and analyzed by flow cytometry using the MACSQuant Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
Before separation | Siglec-F + cells |
Anti-Siglec-F MicroBeads, mouseFigure 2Siglec-F + alveolar macrophages and eosinophils were isolated from mouse lung single-cell suspension using Anti-Siglec-F MicroBeads, two MS Columns, and a MiniMACS Separator. Cells were fluorescently stained with Anti-Siglec-F-APC and CD11c-PE‑Vio ® 770 and analyzed by flow cytometry using the MACSQuant Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. | Anti-Siglec-F MicroBeads, mouseFigure 2Siglec-F + alveolar macrophages and eosinophils were isolated from mouse lung single-cell suspension using Anti-Siglec-F MicroBeads, two MS Columns, and a MiniMACS Separator. Cells were fluorescently stained with Anti-Siglec-F-APC and CD11c-PE‑Vio ® 770 and analyzed by flow cytometry using the MACSQuant Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
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