Anti-Red Blood Cell MicroBeads, rat have been developed for the depletion of rat erythrocytes, e.g., from rat lung, heart, fat, muscle, or spleen as an alternative method to osmotic lysis.

Data and images for Anti-Red Blood Cell MicroBeads, rat

Figures

Figure 1

Red blood cells were depleted from a rat spleen cell suspension using Anti-Red Blood Cell MicroBeads, an LD Column, and a MidiMACS™ Separator. Cells were fluorescently stained with Anti-Erythroid Cells-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before separation
After separation
View details

Figure 1

Red blood cells were depleted from a rat spleen cell suspension using Anti-Red Blood Cell MicroBeads, an LD Column, and a MidiMACS™ Separator. Cells were fluorescently stained with Anti-Erythroid Cells-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Red blood cells were depleted from a rat spleen cell suspension using Anti-Red Blood Cell MicroBeads, an LD Column, and a MidiMACS™ Separator. Cells were fluorescently stained with Anti-Erythroid Cells-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Anti-Red Blood Cell MicroBeads, rat

Overview

Anti-Red Blood Cell MicroBeads, rat have been developed for the depletion of rat erythrocytes, e.g., from rat lung, heart, fat, muscle, or spleen as an alternative method to osmotic lysis.

Detailed product information

Background information

Anti-Red Blood Cell MicroBeads, rat have been developed for the separation of cells expressing a 52 kDa glycophorin A–associated protein, also known as Ly76. It is expressed on rat erythrocytes and erythroid progenitors, but not on granulocytes, monocytes, or lymphocytes.

Columns

LD Columns.

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