The Anti-PSA-NCAM MicroBead kit has been designed for the isolation of PSA-NCAM
+
neural progenitors from differentiated ES or iPS cell cultures.

Data and images for Anti-PSA-NCAM MicroBead Kit, human and mouse

Figures

Figure 1

PSA-NCAM
+
cells were isolated from human pluripotent stem cell-derived neuronal precursor cells using the Anti-PSA-NCAM MicroBead Kit, an LS Column, and a MiniMACS™ Separator. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before separation
PSA-NCAM
+
cells
View details

Figure 1

PSA-NCAM
+
cells were isolated from human pluripotent stem cell-derived neuronal precursor cells using the Anti-PSA-NCAM MicroBead Kit, an LS Column, and a MiniMACS™ Separator. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

PSA-NCAM
+
cells were isolated from human pluripotent stem cell-derived neuronal precursor cells using the Anti-PSA-NCAM MicroBead Kit, an LS Column, and a MiniMACS™ Separator. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Anti-PSA-NCAM MicroBead Kit, human and mouse

Overview

The Anti-PSA-NCAM MicroBead kit has been designed for the isolation of PSA-NCAM
+
neural progenitors from differentiated ES or iPS cell cultures.

Detailed product information

Background information

The Anti-PSA-NCAM antibody recognizes an epitope on polysialic acid (PSA) which, in vertebrates, is found linked to the extracellular domain of the neural cell adhesion molecule (NCAM, CD56).¹ PSA-NCAM, the highly polysialated form of NCAM, is predominantly expressed
in embryonic and neonatal neural tissue.² In vitro differentiation of human and mouse pluripotent stem cells under defined conditions revealed that neuronal progenitors, e.g. long-term self-renewing neuroepithelial-like stem cells, express the surface marker PSA-NCAM.³ Magnetically enriched, in vitro generated PSA-NCAM positive neural progenitors can be further differentiated into defined neuronal subtypes.

Columns

LS or autoMACS
®
Columns.

References for Anti-PSA-NCAM MicroBead Kit, human and mouse

Publications

  1. Barral, S. et al. (2013)
    Efficient neuronal
    in vitro
    and
    in vivo
    differentiation after immunomagnetic purification of mESC derived neuronal precursors.
    Stem Cell Res. 10(2): 133-46