Anti-Mouse IgG1 MicroBeads can be used for magnetic labeling and subsequent separation of cells, which are labeled with a primary mouse IgG1 antibody. They can also be used for positive selection or depletion of mouse B cells expressing IgG1 on the cell surface.

Data and images for Anti-Mouse IgG1 MicroBeads

Figures

Figure 1

Human kidney cell subsets isolated by using an anti-Tamm-Horsefall glycoprotein antibody for distal tubulus cells and a CD13 antibody for proximal tubulus cells in combination with Anti-Mouse IgG1 MicroBeads. (Courtesy of Dr. Baer, Frankfurt a.M., Germany.)
Kidney cells before MACS
®
Separation
Purified distal tubulus cells
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Figure 1

Human kidney cell subsets isolated by using an anti-Tamm-Horsefall glycoprotein antibody for distal tubulus cells and a CD13 antibody for proximal tubulus cells in combination with Anti-Mouse IgG1 MicroBeads. (Courtesy of Dr. Baer, Frankfurt a.M., Germany.)
View details

Figure 1

Human kidney cell subsets isolated by using an anti-Tamm-Horsefall glycoprotein antibody for distal tubulus cells and a CD13 antibody for proximal tubulus cells in combination with Anti-Mouse IgG1 MicroBeads. (Courtesy of Dr. Baer, Frankfurt a.M., Germany.)
Purified proximal tubulus cells
View details

Figure 1

Human kidney cell subsets isolated by using an anti-Tamm-Horsefall glycoprotein antibody for distal tubulus cells and a CD13 antibody for proximal tubulus cells in combination with Anti-Mouse IgG1 MicroBeads. (Courtesy of Dr. Baer, Frankfurt a.M., Germany.)

Specifications for Anti-Mouse IgG1 MicroBeads

Overview

Anti-Mouse IgG1 MicroBeads can be used for magnetic labeling and subsequent separation of cells, which are labeled with a primary mouse IgG1 antibody. They can also be used for positive selection or depletion of mouse B cells expressing IgG1 on the cell surface.

Detailed product information

Downstream applications

Anti-Mouse IgG1 MicroBeads have been used for the isolation of T cells and T cell subpopulations
1
, B cell subsets
2
, rat type I alveolar epithelial cells
3
, and for the isolation of rare cells, such as subsets of kidney cells
4
, ovarian carcinoma cells
5
, or myeloma cells
6
. The isolation of keratinocytes by depletion of non-keratinocytes has also been performed with Anti-Mouse IgG1 MicroBeads.
7

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

Resources for Anti-Mouse IgG1 MicroBeads

References for Anti-Mouse IgG1 MicroBeads

Publications

  1. Jung, T. et al. (1993) Detection of intracellular cytokines by flow cytometry. J. Immunol. Methods 159: 197-207
  2. Näsman et al. (1996) Evidence for oligoclonal diversification of the VH6-containing immunoglobulin repertoire during reconstitution after bone marrow transplantation. Blood 87: 2795-2804
  3. Dobbs, L. G. et al. (1998) Highly water-permeable type I alveolar epithelial cells confer high water permeability between the airspace and vasculature in rat lung. Proc. Natl. Acad. Sci. U.S.A. 95: 2991-2996
  4. Baer, P. C. et al. (1997) Isolation of proximal and distal tubule cells from human kidney by immunomagnetic separation. Technical note. Kidney Int. 52: 1321-1331
  5. Harbeck, N. et al. (1995) Model system for isolation of competent ovarian-carcinoma cells from fresh tumor-tissue by a magnetic separation technique (MACS). Int. J. Oncol. 6: 1249-1254
  6. Drach et al. (1995)
    Multiple myeloma: high incidence of chromosomal aneuploidy as detected by interphase fluorescence
    in situ
    hybridization.
    Cancer Res. 55: 3854-3859
  7. Formanek et al. (1998) Magnetic cell separation for purification of human oral keratinocytes: an effective method for functional studies without prior cell subcultivation. Eur. Arch. Otorhinolaryngol. 255: 211-215

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