Anti-iNKT MicroBeads have been developed for the positive selection of human invariant natural killer T cells (iNKT cells) based on the expression of the TCR α-chain Vα24-Jα18.

Data and images for Anti-iNKT MicroBeads, human

Figures

Figure 1

The iNKT cells were isolated from human PBMCs using Anti-iNKT MicroBeads, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-iNKT-PE, CD3-APC, and CD45-FITC and analyzed using the MACSQuant® Analyzer. Gating was performed according to CD45 expression and side scatter properties of the cells.
Unseparated fraction
After separation
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Figure 1

The iNKT cells were isolated from human PBMCs using Anti-iNKT MicroBeads, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-iNKT-PE, CD3-APC, and CD45-FITC and analyzed using the MACSQuant® Analyzer. Gating was performed according to CD45 expression and side scatter properties of the cells.
View details

Figure 1

The iNKT cells were isolated from human PBMCs using Anti-iNKT MicroBeads, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-iNKT-PE, CD3-APC, and CD45-FITC and analyzed using the MACSQuant® Analyzer. Gating was performed according to CD45 expression and side scatter properties of the cells.

Specifications for Anti-iNKT MicroBeads, human

Overview

Anti-iNKT MicroBeads have been developed for the positive selection of human invariant natural killer T cells (iNKT cells) based on the expression of the TCR α-chain Vα24-Jα18.

Detailed product information

Background information

iNKT cells represent a distinct lymphocyte population, characterized by expression of an invariant T cell receptor α-chain and certain TCR β-chains (Vα24-Jα18 combined with Vβ11). iNKT cells are involved in immunoregulatory processes such as tolerance, host defense, and tumor surveillance.
Due to the nature of the antibody, a limited degree of pre-activation of target cells might occur after separation with this product.

Columns

MS, LS, XS, or autoMACS
®
Columns.

Resources for Anti-iNKT MicroBeads, human

Documents and Protocols

References for Anti-iNKT MicroBeads, human

Publications

  1. Exley, M. et al. (2008) Selective activation, expansion, and monitoring of human iNKT cells with a monoclonal antibody specific for the TCR α-chain CDR3 loop. Eur. J. Immunol. 38: 1756-1766
  2. Montoya, J. C. et al. (2007) Characterization of human invariant natural killer T subsets in health and disease using a novel invariant natural killer T cell-clonotypic monoclonal antibody, 6B11. Immunology 122: 1-14

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