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IgG + memory B cells were separated from PBMCs. CD19 + B cells were isolated using the CD19 MultiSort Kit. Fc receptor-bound IgG was removed by washing with sodium acetate buffer. Memory B cells were isolated by using Anti-IgG MicroBeads, an MS Column, and a MiniMACS™ Separator. |
Pre-selected CD19 + cells | Isolated surface IgG + memory B cells |
Anti-IgG MicroBeads, humanFigure 1IgG + memory B cells were separated from PBMCs. CD19 + B cells were isolated using the CD19 MultiSort Kit. Fc receptor-bound IgG was removed by washing with sodium acetate buffer. Memory B cells were isolated by using Anti-IgG MicroBeads, an MS Column, and a MiniMACS™ Separator. | Anti-IgG MicroBeads, humanFigure 1IgG + memory B cells were separated from PBMCs. CD19 + B cells were isolated using the CD19 MultiSort Kit. Fc receptor-bound IgG was removed by washing with sodium acetate buffer. Memory B cells were isolated by using Anti-IgG MicroBeads, an MS Column, and a MiniMACS™ Separator. |
IgG + memory B cells were separated from PBMCs. CD19 + B cells were isolated using the CD19 MultiSort Kit. Fc receptor-bound IgG was removed by washing with sodium acetate buffer. Memory B cells were isolated by using Anti-IgG MicroBeads, an MS Column, and a MiniMACS™ Separator. |
Pre-selected CD19 + cells | Isolated surface IgG + memory B cells |
Anti-IgG MicroBeads, humanFigure 1IgG + memory B cells were separated from PBMCs. CD19 + B cells were isolated using the CD19 MultiSort Kit. Fc receptor-bound IgG was removed by washing with sodium acetate buffer. Memory B cells were isolated by using Anti-IgG MicroBeads, an MS Column, and a MiniMACS™ Separator. | Anti-IgG MicroBeads, humanFigure 1IgG + memory B cells were separated from PBMCs. CD19 + B cells were isolated using the CD19 MultiSort Kit. Fc receptor-bound IgG was removed by washing with sodium acetate buffer. Memory B cells were isolated by using Anti-IgG MicroBeads, an MS Column, and a MiniMACS™ Separator. |
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