Anti-Fibroblast MicroBeads have been developed for the positive selection or depletion of human fibroblasts.

Data and images for Anti-Fibroblast MicroBeads, human

Figures

Figure 1

Positive selection of fibroblasts from a mixture of cells of an epithelial tumor cell line and normal human dermal fibroblasts using Anti-Fibroblast MicroBeads, an MS Column, and a MiniMACS™ Separator.
Unseparated fraction
Non-fibroblast cells in flow-through
View details

Figure 1

Positive selection of fibroblasts from a mixture of cells of an epithelial tumor cell line and normal human dermal fibroblasts using Anti-Fibroblast MicroBeads, an MS Column, and a MiniMACS™ Separator.
View details

Figure 1

Positive selection of fibroblasts from a mixture of cells of an epithelial tumor cell line and normal human dermal fibroblasts using Anti-Fibroblast MicroBeads, an MS Column, and a MiniMACS™ Separator.
Isolated fibroblasts
View details

Figure 1

Positive selection of fibroblasts from a mixture of cells of an epithelial tumor cell line and normal human dermal fibroblasts using Anti-Fibroblast MicroBeads, an MS Column, and a MiniMACS™ Separator.

Specifications for Anti-Fibroblast MicroBeads, human

Overview

Anti-Fibroblast MicroBeads have been developed for the positive selection or depletion of human fibroblasts.

Detailed product information

Background information

Anti-Fibroblast MicroBeads are based on a fibroblast-specific antigen.
1
This antigen is also known to be expressed on mesenchymal stem cells, also known as mesenchymal stromal cells, (MSCs) in the bone marrow.
2–4

Applications

  • Depletion of human fibroblasts that are used as feeder cells to support the growth of ES or iPS cells
  • Depletion of fibroblasts after reprogramming5
  • Isolation of MSCs from bone marrow aspirate
  • Removal of fibroblastic cells from bone marrow cultures
  • Positive selection of fibroblasts from cell mixtures

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

References for Anti-Fibroblast MicroBeads, human

Publications

  1. Fearns, C. and Dowdle, E. B. (1992)
    The desmoplastic response: induction of collagen synthesis by melanoma cells
    in vitro
    .
    Int. J. Cancer 50: 621-627
  2. Jones, E. A. et al. (2002) Isolation and characterization of bone marrow multipotential mesenchymal progenitor cells. Arthritis Rheum. 46: 3349-3360
  3. Jones et al. (2006) Optimization of a flow cytometry-based protocol for detection and phenotypic characterization of multipotent mesenchymal stromal cells from human bone marrow. Cytometry B Clin. Cytom. 70: 391-399
  4. Jones et al. (2007) MACS&more 11(1): 22-25
  5. Paul, D. et al. (2015) Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells. Nat. Methods

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