Anti-DC (OX62) MicroBeads have been developed for the positive selection or depletion of OX62
+
dendritic cells (DCs) from blood, lymphoid, and non-lymphoid tissue.

Data and images for Anti-DC (OX62) MicroBeads, rat

Figures

Figure 1

Isolation of dendritic cells from rat spleen using Anti-DC (OX62) MicroBeads, a MiniMACS™ Separator, and an MS Column. Isolated cells were stained with Anti-MHC class II (OX6)-FITC, CD4-PE, and Anti-DC (OX62)-APC.
A:
B:
Spleen cells before separation
Isolated dendritic cells
View details

Figure 1

Isolation of dendritic cells from rat spleen using Anti-DC (OX62) MicroBeads, a MiniMACS™ Separator, and an MS Column. Isolated cells were stained with Anti-MHC class II (OX6)-FITC, CD4-PE, and Anti-DC (OX62)-APC.
View details

Figure 1

Isolation of dendritic cells from rat spleen using Anti-DC (OX62) MicroBeads, a MiniMACS™ Separator, and an MS Column. Isolated cells were stained with Anti-MHC class II (OX6)-FITC, CD4-PE, and Anti-DC (OX62)-APC.
C:
Isolated dendritic cells
View details

Figure 1

Isolation of dendritic cells from rat spleen using Anti-DC (OX62) MicroBeads, a MiniMACS™ Separator, and an MS Column. Isolated cells were stained with Anti-MHC class II (OX6)-FITC, CD4-PE, and Anti-DC (OX62)-APC.

Figure 2

View details
Isolated OX62
+
dendritic cells from rat spleen, May-Grünwald/Giemsa stained. (Courtesy of Dr. R. Josien, Nantes, France.)

Figure 2

Isolated OX62
+
dendritic cells from rat spleen, May-Grünwald/Giemsa stained. (Courtesy of Dr. R. Josien, Nantes, France.)

Specifications for Anti-DC (OX62) MicroBeads, rat

Overview

Anti-DC (OX62) MicroBeads have been developed for the positive selection or depletion of OX62
+
dendritic cells (DCs) from blood, lymphoid, and non-lymphoid tissue.

Detailed product information

Background information

The monoclonal antibody OX62 recognizes the integrin αE2 subunit (molecular mass: 150 kDa). OX62 is expressed on the
CD4
+
and the CD4
-
subset of dendritic cells but not on plasmacytoid dendritic cells in lymphoid tissue or on epidermal Langerhans cells. OX62 is also found on a subpopulation of epidermal TCRα/β
+
or TCRγ/δ
+
T cells and on a subset of folliculo-stellate-like (S100
+
) cells in the anterior pituitary. It is not expressed on macrophages.

Downstream applications

Isolated OX62
+
dendritic cells were used, for example, for analysis of the differential phenotype, function, and migration of dendritic cell subsets.
1

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

References for Anti-DC (OX62) MicroBeads, rat

Publications

  1. Kobayashi, H. et al. (2004)
    In situ
    demonstration of dendritic cell migration from rat intestine to mesenteric lymph nodes: relationships to maturation and role of chemokines.
    J. Leukoc. Biol. 75: 434-442