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Human carcinoma cells were purified from a mixture of dissociated FFPE tumor cells spiked in dissociated FFPE spleen cells using Anti-Cytokeratin MicroBeads, human. Isolation was performed on an autoMACS ® Pro Separator. The cells were fluorescently stained with Anti-Cytokeratin-FITC, Anti-Vimentin-APC, and CD235a (Glycophorin A)-PE, and DAPI and analyzed by flow cytometry using the MACSQuant ® Analyzer. Aggregates were excluded from the analysis based on scatter signals and single cells were gated on DAPI fluorescence. |
Before separation | After separation |
Anti-Cytokeratin MicroBeads, humanFigure 1Human carcinoma cells were purified from a mixture of dissociated FFPE tumor cells spiked in dissociated FFPE spleen cells using Anti-Cytokeratin MicroBeads, human. Isolation was performed on an autoMACS ® Pro Separator. The cells were fluorescently stained with Anti-Cytokeratin-FITC, Anti-Vimentin-APC, and CD235a (Glycophorin A)-PE, and DAPI and analyzed by flow cytometry using the MACSQuant ® Analyzer. Aggregates were excluded from the analysis based on scatter signals and single cells were gated on DAPI fluorescence. | Anti-Cytokeratin MicroBeads, humanFigure 1Human carcinoma cells were purified from a mixture of dissociated FFPE tumor cells spiked in dissociated FFPE spleen cells using Anti-Cytokeratin MicroBeads, human. Isolation was performed on an autoMACS ® Pro Separator. The cells were fluorescently stained with Anti-Cytokeratin-FITC, Anti-Vimentin-APC, and CD235a (Glycophorin A)-PE, and DAPI and analyzed by flow cytometry using the MACSQuant ® Analyzer. Aggregates were excluded from the analysis based on scatter signals and single cells were gated on DAPI fluorescence. |
Human carcinoma cells were purified from a mixture of dissociated FFPE tumor cells spiked in dissociated FFPE spleen cells using Anti-Cytokeratin MicroBeads, human. Isolation was performed on an autoMACS ® Pro Separator. The cells were fluorescently stained with Anti-Cytokeratin-FITC, Anti-Vimentin-APC, and CD235a (Glycophorin A)-PE, and DAPI and analyzed by flow cytometry using the MACSQuant ® Analyzer. Aggregates were excluded from the analysis based on scatter signals and single cells were gated on DAPI fluorescence. |
Before separation | After separation |
Anti-Cytokeratin MicroBeads, humanFigure 1Human carcinoma cells were purified from a mixture of dissociated FFPE tumor cells spiked in dissociated FFPE spleen cells using Anti-Cytokeratin MicroBeads, human. Isolation was performed on an autoMACS ® Pro Separator. The cells were fluorescently stained with Anti-Cytokeratin-FITC, Anti-Vimentin-APC, and CD235a (Glycophorin A)-PE, and DAPI and analyzed by flow cytometry using the MACSQuant ® Analyzer. Aggregates were excluded from the analysis based on scatter signals and single cells were gated on DAPI fluorescence. | Anti-Cytokeratin MicroBeads, humanFigure 1Human carcinoma cells were purified from a mixture of dissociated FFPE tumor cells spiked in dissociated FFPE spleen cells using Anti-Cytokeratin MicroBeads, human. Isolation was performed on an autoMACS ® Pro Separator. The cells were fluorescently stained with Anti-Cytokeratin-FITC, Anti-Vimentin-APC, and CD235a (Glycophorin A)-PE, and DAPI and analyzed by flow cytometry using the MACSQuant ® Analyzer. Aggregates were excluded from the analysis based on scatter signals and single cells were gated on DAPI fluorescence. |
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