Anti-AN2 MicroBeads have been developed for positive selection or depletion of murine or human AN2
+
glial cells.

Data and images for Anti-AN2 MicroBeads, human and mouse

Figures

Figure 1

AN2
+
cells were isolated, after re-expression of the AN2 antigen, from day 6 postnatal CD1 mouse brain tissue using the Neural Tissue Dissociation Kit (P), the gentleMACS™ Dissociator, FcR Blocking Reagent, mouse, the Anti-AN2 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with Labeling Check Reagent-PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before separation
AN2
cells
View details

Figure 1

AN2
+
cells were isolated, after re-expression of the AN2 antigen, from day 6 postnatal CD1 mouse brain tissue using the Neural Tissue Dissociation Kit (P), the gentleMACS™ Dissociator, FcR Blocking Reagent, mouse, the Anti-AN2 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with Labeling Check Reagent-PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

AN2
+
cells were isolated, after re-expression of the AN2 antigen, from day 6 postnatal CD1 mouse brain tissue using the Neural Tissue Dissociation Kit (P), the gentleMACS™ Dissociator, FcR Blocking Reagent, mouse, the Anti-AN2 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with Labeling Check Reagent-PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
AN2
+
cells
View details

Figure 1

AN2
+
cells were isolated, after re-expression of the AN2 antigen, from day 6 postnatal CD1 mouse brain tissue using the Neural Tissue Dissociation Kit (P), the gentleMACS™ Dissociator, FcR Blocking Reagent, mouse, the Anti-AN2 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with Labeling Check Reagent-PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Anti-AN2 MicroBeads, human and mouse

Overview

Anti-AN2 MicroBeads have been developed for positive selection or depletion of murine or human AN2
+
glial cells.

Detailed product information

Background information

AN2
1
is the mouse homolog of the rat NG2 and human MCSP protein. NG2 is a chondroitin sulfate glycoprotein that is expressed by glial progenitor cells in the developing and adult central nervous system (CNS) and by immature Schwann cells.
NG2/AN2
+
glia has been identified as a fourth major glial cell type in the mammalian CNS that is distinct from other cell types
2
. NG2/AN2
+
cells are precursors of oligodendrocytes and some protoplasmic astrocytes in the grey matter
3
. NG2/AN2 expression is down-regulated before the cells undergo terminal differentiation. However, a population of NG2/AN2
+
cells remain in the white and grey matter after oligodendrocytes are generated
4-6
.
NG2/AN2
+
glial cells participate in neuron-glial circuits by receiving presynaptic input from neurons and responding to neurotransmitters released by synapses
7
.
Many highly migratory neural tumors including melanomas express NG2/AN2.

Columns

For positive selection: MS, LS, or autoMACS
®
Columns. For depletion: LD or autoMACS Columns.

Resources for Anti-AN2 MicroBeads, human and mouse

References for Anti-AN2 MicroBeads, human and mouse

Publications

  1. Stegmuller, J. et al. (2002) AN2, the mouse homologue of NG2, is a surface antigen on glial precursor cells implicated in control of cell migration. J. Neurocytol. 31: 497-505
  2. Trotter, J. et al. (2010) NG2 cells: Properties, progeny and origin. Brain Res. Rev. 63: 72-82
  3. Zhu, X. et al. (2008) NG2 cells generate both oligodendrocytes and gray matter astrocytes. Development 132: 145-157
  4. Diers-Fenger, M. et al. (2001) AN2/NG2 protein-expressing glial progenitor cells in the murine CNS: isolation, differentiation, and association with radial glia. Glia 34: 213-228
  5. Peters, A. (2004) A fourth type of neuroglial cell in the adult central nervous system. J. Neurocytol. 33: 345-357
  6. Zhu, X. et al. (2011) Age-dependent fate and lineage restriction of single NG2 cells. Development 138: 745-753
  7. Wigley, R. et al. (2007) Morphological and physiological interactions of NG2-glia with astrocytes and neurons. J. Anat. 210: 661-670
  8. Nishiyama, A. et al. (2009) Polydendrocytes (NG2 cells): multifunctional cells with lineage plasticity. Nat. Rev. Neurosci. 10: 9-22

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