Clone:
REA881
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC
Alternative names:
IKZF3, Znfn1a3

Extended validation for Aiolos Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA881
14C4C97-
16D9C97-
S50-895++
Cells were incubated with an excess of purified unconjugated Aiolos (REA881) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for Aiolos. Human peripheral blood mononuclear cells (PBMCs) were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Transcription Factor Staining Buffer Set followed by intracellular staining with Aiolos antibodies. As a control, Aiolos antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Aiolos. Human peripheral blood mononuclear cells (PBMCs) were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Transcription Factor Staining Buffer Set followed by intracellular staining with Aiolos antibodies. As a control, Aiolos antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Aiolos. Human peripheral blood mononuclear cells (PBMCs) were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Transcription Factor Staining Buffer Set followed by intracellular staining with Aiolos antibodies. As a control, Aiolos antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for Aiolos. Human peripheral blood mononuclear cells (PBMCs) were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Transcription Factor Staining Buffer Set followed by intracellular staining with Aiolos antibodies. As a control, Aiolos antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for Aiolos Antibody, anti-human, REAfinity™

Overview

Clone REA881 recognizes the human transcription factor aiolos, also known as IKZF3. Aiolos is a member of the ikaros family of zinc-finger proteins and forms heterodimers with other ikaros family members. It plays an essential role in the control of B lymphocyte differentiation and proliferation and in the regulation of BCL2 expression. Furthermore, it is involved in controlling apoptosis in T cells in an IL-2–dependent manner. Aiolos is found in peripheral blood leukocytes, spleen, and thymus.
Additional information: Clone REA881 displays negligible binding to Fc receptors.

Alternative names

IKZF3, Znfn1a3

Detailed product information

Technical specifications

CloneREA881
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenAiolos
Alternative names of antigenIKZF3, Znfn1a3
Molecular mass of antigen [kDa]58
Distribution of antigenleukocytes, spleen
Entrez Gene ID22806
RRIDAB_2726621, AB_2726552, AB_2726622, AB_2726553, AB_2726551, AB_2726620

References for Aiolos Antibody, anti-human, REAfinity™

Publications

  1. Romero, F. et al. (1999) Aiolos transcription factor controls cell death in T cells by regulating Bcl-2 expression and its cellular localization. EMBO J. 18(12): 3419-3430
  2. Caballero, R. et al. (2007) Combinatorial effects of splice variants modulate function of Aiolos. J. Cell. Sci. 120(15): 2619-2630
  3. Billot, K. et al. (2010) Differential aiolos expression in human hematopoietic subpopulations. Leuk. Res. 34(3): 289-293

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