The Adult Neuron Isolation Kit, mouse depletes all non-neuronal cells to acquire highly purified neurons from mouse brain tissue older than postnatal day 7, resulting in superior performance in single cell sequencing and cell culture.

Data and images for Adult Neuron Isolation Kit, mouse

Figures

Figure 1

Neuronal cells were isolated from brains of seven weeks-old CD-1 mice. Adult mouse brains were dissociated using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Adult Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, the microglia-specific antibody CD11b-PE and the endothelial cell-specific antibody CD31-PE. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before separation
After separation
View details

Figure 1

Neuronal cells were isolated from brains of seven weeks-old CD-1 mice. Adult mouse brains were dissociated using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Adult Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, the microglia-specific antibody CD11b-PE and the endothelial cell-specific antibody CD31-PE. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Neuronal cells were isolated from brains of seven weeks-old CD-1 mice. Adult mouse brains were dissociated using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Adult Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, the microglia-specific antibody CD11b-PE and the endothelial cell-specific antibody CD31-PE. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Neuronal cells were isolated from brains of seven weeks-old CD-1 mice. Adult mouse brains were dissociated using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Adult Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, the microglia-specific antibody CD11b-PE and the endothelial cell-specific antibody CD31-PE. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Neuronal cells were isolated from brains of seven weeks-old CD-1 mice. Adult mouse brains were dissociated using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Adult Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, the microglia-specific antibody CD11b-PE and the endothelial cell-specific antibody CD31-PE. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Neuronal cells were isolated from brains of seven weeks-old CD-1 mice. Adult mouse brains were dissociated using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Adult Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, the microglia-specific antibody CD11b-PE and the endothelial cell-specific antibody CD31-PE. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Neuronal cells were isolated from brains of seven weeks-old CD-1 mice. Adult mouse brains were dissociated using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Adult Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, the microglia-specific antibody CD11b-PE and the endothelial cell-specific antibody CD31-PE. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Neuronal cells were isolated from brains of seven weeks-old CD-1 mice. Adult mouse brains were dissociated using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Adult Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, the microglia-specific antibody CD11b-PE and the endothelial cell-specific antibody CD31-PE. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Neuronal cells were isolated from brains of seven weeks-old CD-1 mice. Adult mouse brains were dissociated using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Adult Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, the microglia-specific antibody CD11b-PE and the endothelial cell-specific antibody CD31-PE. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Adult Neuron Isolation Kit, mouse

Overview

The Adult Neuron Isolation Kit, mouse depletes all non-neuronal cells to acquire highly purified neurons from mouse brain tissue older than postnatal day 7, resulting in superior performance in single cell sequencing and cell culture.

Detailed product information

Background information

The Adult Neuron Isolation Kit, mouse is an indirect magnetic labeling system for the isolation of untouched neurons from cell suspensions of mouse neural tissue older than postnatal day 7 (P7). Non-neuronal cells like astrocytes, oligodendrocytes, microglia, endothelial cells, fibroblasts, except erythrocytes, are indirectly magnetically labeled by using biotin-conjugated antibodies specific for non-neuronal cells in combination with Anti-Biotin MicroBeads. Isolation of highly pure unlabeled neuronal cells is achieved by depletion of the magnetically labeled cells.
The cell number and composition of the neuronal cell fraction varies with the mouse age and the brain region used for cell isolation.
For optimal results, it is recommended to use the Adult Brain Dissociation Kit, mouse and rat (# 130-107-677) prior to this kit.

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