A complete workflow for activation and expansion of your T cells

Optimize ex vivo T cell activation and expansion in translational research, while preserving functionality and viability. After gentle T cell isolation with our MACS® Technology solutions, activation can be either performed polyclonally or antigen-specific. Efficient and reliable proliferation ensures viable and functional T cells for any downstream application. 

Our flow cytometry solutions enable highly accurate multiparameter T cell analysis.


Application data by workflow step

T cell isolation

Streamlined magnetic T cell isolation with MACS® Technology

Reliability now comes with better performance and faster protocols. Our improved MicroBeads and T Cell Isolation Kits allow for high-quality separation of  human and mouse T cells within a few minutes. 

Comparison of activation marker expression of mouse CD4+ and CD8+ T cells isolated with MACS MicroBeads or T Cell Isolation Kits
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MACS MicroBeads

  • Updated CD4, CD8a, CD90.1 and CD90.2 MicroBeads, mouse allow for positive selection of T cells in only 13.5 minutes

  • MicroBeads are available with many human, mouse, rat and non-human primate specificities

T cell isolation kits

  • Untouched isolation of human and mouse T cells and T cell subsets in as little as 18 minutes

T cell isolation directly from blood products

StraightFrom® MicroBeads allow magnetic cell separation directly from human blood products, such as whole blood, buffy coat, leukoreduction system chambers (LRSCs) and leukopaks. CD4+ and CD8+ T cell subsets can be purified using the autoMACS® Pro Separator, the MultiMACS™ Cell24 Separator Plus, or manual separators.


Comparison of StraightFrom Buffy Coat MicroBeads with other commercially available products
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StraightFrom® MicroBeads 

  • No density gradient centrifugation, no erythrocyte lysis
  • Easy and fast protocols with only a few handling steps
  • No cell activation and no effects on cell biology


Label-free T cell isolation

REAlease® Technology allows for magnetic cell isolation with subsequent removal of any beads and labels from your cells of interest. Clever utilization of recombinant antibody fragments allows for cell surface labeling in just a few steps. 

The technology relies on recombinantly engineered antibody fragments for cell surface labeling for highly reproducible results. The REAlease Biotin Complex binds to the target cells. Labeling of the REAlease Biotin Complex with Anti-Biotin MicroBeads allows for magnetic isolation of these cells. Following cell separation, both MicroBeads and REAlease Biotin Complex are gently removed, leaving the cells bead-and label-free. Enriched cells are then suitable for magnetic re-labeling and any application where label-free cells are essential.

Principle of REAlease MicroBead technology
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REAlease MicroBeads

  • Bead-free cells suited for a second positive selection

  • Label-free cells for any downstream application

  • Recombinant antibody fragments for reproducible results
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Scientific poster
REAlease Immunomagnetic Separation Technology with reversible labeling for postive selection of leukocytes

Philipp Steinbrueck, Zhong Yu, Ursula Bissa, Jennifer Brieden, and Christian Dose, Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany


T cell culture

T cell medium

TexMACS™ Medium is an optimized, serum-free cell culture medium developed for reliable T cell and Treg cell culture results. Chemically-defined and without animal-derived components, it allows for the highest reproducibility in human and mouse cell culture applications.

Comparison of expansion rates of human CD4+ and CD8+ T cells in TexMACS Medium or competitor’s T cell medium using the T Cell Activation/Expansion Kit, human
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TexMACS™ Medium

  • Free of serum and other animal-derived components
  • Reliable T cell activation and high expansion rates
  • Up to MACS® GMP grade
  • Available with or without phenol red

T cell activation and expansion

We offer a variety of polyclonal and antigen-specific T cell stimulation tools for you to choose from. Find the options below:

 Expansion of human and mouse CD4+ and CD8+ T cells using T Cell Activation/Expansion Kits
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T cell Activation/Expansion Kits 

  • MACSiBead™ Particles loaded with functional-grade antibodies
  • Efficient activation and expansion of human, mouse, and non-human primate T cells or Treg cells
Activation and expansion of CD4+ and CD8+ T cells with T Cell TransAct
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T Cell TransAct™ Stimulation Reagent

  • Ready-to-use reagent for activation and expansion of human T cells
  • Simple volumetric dosage and easy removal by washing
  • Up to MACS® GMP grade
Comparison of T cell activation using the CytoStim Reagent, SEB, or PMA/Ionomycin
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CytoStim™ Reagent

  • Non-toxic alternative to the superantigen SEB for the stimulation of human T cells
Production of IFN-γ by human CD4+ or CD8+ T cells after stimulation with different antigens using PepTivator Peptide Pools
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PepTivator® Peptide Pools

  • Stimulation of both CD4+ and CD8+ T cells with our extensive panel of antigens
  • 15-mer peptides with 11-amino acid overlap that cover the complete sequence of the respective antigen
  • Available in 96-well format
  • MACS GMP-grade peptide pools for translational settings


Multiparameter flow analysis of T cells

As the final step in many workflows, flow analysis plays an important part in various T cell applications. The following flow reagents can help you make sure that the data you generate on your flow cytometer are reliable and consistent:

  • REAfinity™ Recombinant Antibodies recombinantly generated antibodies that ensure lot-to-lot consistency and eliminate background signal
  • MACSplex™ Cytokine Kits analysis of multiple soluble cytokines in one sample using any standard flow cytometer
  • Rapid Cytokine Inspector – combined surface marker and intracellular cytokine staining for multiparameter analysis of activated human T cells
  • Cytokine Secretion Assay – Detection Kits – flow analysis of cytokine secretion at a detection level of one T cell in a million
  • Build your own T cell flow panel with our Flow Panel Builder 

Related information


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