PB buffer: Prepare a solution containing phosphate-buffered saline (PBS), pH 7.2, and 0.5% bovine serum albumin (BSA) by diluting MACS® BSA Stock Solution 1:20 with PBS. Keep buffer cold (2−8 °C). Prepare fresh on the day of use and degas the buffer, as air bubbles could block the column.
▲ Note: BSA can be replaced by other proteins such as mouse serum albumin, mouse serum, or fetal bovine serum (FBS).
Coat a 24-well culture dish with 0.01% Poly-L-lysine overnight at 37 °C. Wash three times with ddH₂O the following day.
Prepare the following cell culture medium: MACS Neuro Medium containing 2% MACS NeuroBrew®-21, 1% penicillin/streptomycin and 0.5 mM L-glutamine, 10 ng/mL Human PDGF-AA, and 10 ng/mL Human FGF-2.
Dissociate mouse neonatal brain using the Neural Tissue Dissociation Kit (P) or Neural Tissue Dissociation Kit (T). Follow the protocol of the kit data sheet.
Download data sheet
Isolate oligodendrocyte precursor cells using the CD140a (PDGFRα) MicroBead Kit, mouse. Follow the protocol of the kit data sheet.
Download data sheet
To stain and analyze the isolated oligodendrocyte precursor cells by flow cytometry, follow the protocol of the Labeling Check Reagent.
Column | Max. number of labeled cells | Max. number of total cells | Separator |
---|---|---|---|
Positive selection | |||
MS | 1×107 | 2×107 | MiniMACS™, OctoMACS™, VarioMACS, SuperMACS II |
LS | 2×107 | 4×107 | MidiMACS™, QuadroMACS™, VarioMACS, SuperMACS II |
autoMACS | 5×107 | 1×108 | autoMACS Pro |
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