Miltenyi Biotec-tested panel 26 (MBTP 26)
This application protocol describes the simultaneous quantification of intracellular and surface markers for easy enumeration of regulatory T cells from human PBMCs using flow cytometry.
Specificity | Clone | Purpose | Fluorochrome | Detection filter (nm) (laser) |
CD45 | REA747 | Lymphocytes | VioBlue® | 450/50 (violet) |
CD4 | REA623 | T helper cells | VioGreen™ | 525/50 (violet) |
CD25 | REA570 | Regulatory T cells | Vio® Bright B515 | 525/50 (blue) |
CD127 | REA614 | Regulatory T cells | PE | 585/40 (blue) |
FoxP3 | REA944 | Transcription factor of regulatory T cells | Vio 667 | 655-730 (blue) |
Specificity | Clone | Purpose | Fluorochrome | Detection filter (nm) (laser) |
CD45 | REA747 | Lymphocytes | VioBlue® | 450/50 (violet) |
CD4 | REA623 | T helper cells | VioGreen™ | 525/50 (violet) |
CD25 | REA570 | Regulatory T cells | Vio® Bright B515 | 525/50 (blue) |
CD127 | REA614 | Regulatory T cells | PE | 585/40 (blue) |
FoxP3 | REA944 | Transcription factor of regulatory T cells | Vio 667 | 655-730 (blue) |
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