Slider Image

July 11–15, 2020
Meet us at the FENS 2020 Virtual Forum

Since the last FENS Forum of Neuroscience in 2018, Miltenyi Biotec has witnessed the expansion of a great number of new and exciting technologies which remarkably contributed to the field of neuroscience. From adult brain tissue dissociation to neural cell isolation, cell analysis, and cell cultivation, as well as from easy sample preparation for 3D imaging to fully automated light sheet microscopy, our experts look forward to meeting you in our virtual booth and sharing the latest technologies. Furthermore, don’t miss our Bridging the Knowledge Session on “Holistic whole-brain imaging – from easy sample preparation to high-resolution light sheet microscopy” which will be accessible to FENS 2020 Virtual Forum attendees for 3 months.

Bridging the Knowledge Session

Holistic whole-brain imaging – from easy sample preparation to high-resolution light sheet microscopy.

Date & time

 Sunday, July 12, 2020 | 9:30 a.m.–10:30 a.m. CEST

Speakers & abstracts

Multiparameter immunofluorescent analysis and light sheet microscopy: from high-content 2D to 3D imaging in neuroscience

Melanie Jungblut, Ph.D.
Melanie Jungblut studied biology and chemistry at the University of Kaiserslautern, Germany. She received her Ph.D. in Neurobiology/Biotechnology at the Max Planck Institute for Polymer Research and the University of Mainz, Germany. In 2007, she joined Miltenyi Biotec as a postdoc. Since 2011, she is group leader of Research & Development (R&D), responsible for product development in the field of neuroscience.

The generation of a detailed cellular topography and protein expression cartography is often essential to gain a detailed understanding of cellular and molecular processes.  
For highly complex analyses of cellular compositions and expression patterns, we developed the MACSima™ Imaging Platform, which allows ultrahigh-content imaging based on cyclic immunofluorescent labeling. The system enables fully automated, multiparameter fluorescence imaging for comprehensive protein expression analysis using an automated analysis pipeline.  
To further understand the cellular composition of tissues and organs, sample clearing in combination with light sheet fluorescence microscopy emerged as a powerful imaging tool enabling 3D reconstruction of entire biological systems and processes. We developed a novel procedure based on non-toxic reagents that allows efficient clearing of mouse brain and various organs, as well as stem cell–derived brain organoids. 
Taking advantage of our UltraMicroscope Platforms and  whole-mount immunostaining using novel recombinant antibodies coupled to photostable Vio® Dyes, the process enables reconstruction of cellular maps, thus paving the way towards a better understanding of tissue structure and disease mechanisms.    

 

Functional imaging of interneuron influence on monosynaptically connected place cells in CA1

Tristan Geiller, Ph.D. 
Tristan Geiller is a postdoctoral researcher at Columbia University’s Zuckerman Institute in New York, USA. The goal of his research is to understand the functional architecture of hippocampal circuits which support episodic memory. His approach covers a wide range of techniques from light sheet microscopy for whole-brain imaging to subcellular two-photon imaging in awake-behaving mice.  

The mammalian hippocampus has long been recognized to play a crucial role in learning and memory, which has motivated widespread research efforts to characterize the different hippocampal cell types and determine how they interact to generate neural activity patterns. During spatial behavior, hippocampal place cells can selectively respond to specific locations in an environment. Yet, little is known about the role of local inhibitory networks on supporting these representations. Here, I will present a combination of techniques that allowed us to perform large-scale 3D two-photon imaging of molecularly-identified interneurons in CA1 in awake-behaving mice, and identify those that are monosynaptically connected to a single place cell. Using this monosynaptic tracing technique, we performed tissue clearing followed by whole-brain imaging and reconstruction using the Miltenyi Biotec UltraMicroscope to reveal the location of local and long-range presynaptic neurons, allowing a detailed anatomical mapping at the single-cell level.

Open Theater

Whole-brain imaging at subcellular resolution – introducing the UltraMicroscope Blaze.

Time

Pre-recorded session available on demand at FENS 2020 Virtual Forum.

Description

The lack of technologies that can do large-scale imaging at high resolution remains a major obstacle for deciphering the structural complexity of the central and peripheral nervous system. In this presentation we introduce the UltraMicroscope Blaze which addresses these challenges. This new and automated light sheet microscope enables imaging of multiple organs such as whole brains or large samples like a whole cleared mouse model. With cutting-edge light sheet optics the system bridges the gap between high-resolution imaging of tissue sections and large-scale imaging of whole animals. This allows the microscope to operate as a virtual microtome that can visualize large samples in three dimensions with subcellular resolution. 

Virtual booth

Visit our virtual booth to discover our technologies and to learn more about the adult 
brain workflow, our most popular neuroscience application in the past two years.  
Do you have a question? Simply use the chat function within the booth. 

Cutting-edge technologies for 
your neuroscience research

Neuroscience applications
Applications for neural cell research in vitro

Light sheet microscopy
Multiparametric cell sorting in a closed cartridge system

Tissue clearing reagents
Immunostaining and clearing of neural tissues for optimal 3D imaging analysis

Related information