StraightFrom
®
Whole Blood CD66b MicroBeads were developed for rapid positive selection of CD66b
+
cells directly from human whole blood. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis.

Data and images for
StraightFrom
®
Whole Blood CD66b MicroBeads
, human

Figures

Figure 1

Separation of a whole blood sample using the StraightFrom
®
Whole Blood CD66b MicroBeads and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns without washing step after labeling. Cells were fluorescently stained with CD15‑FITC, CD66b-PE, as well as CD45‑VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before separation
After separation
View details

Figure 1

Separation of a whole blood sample using the StraightFrom
®
Whole Blood CD66b MicroBeads and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns without washing step after labeling. Cells were fluorescently stained with CD15‑FITC, CD66b-PE, as well as CD45‑VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Separation of a whole blood sample using the StraightFrom
®
Whole Blood CD66b MicroBeads and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns without washing step after labeling. Cells were fluorescently stained with CD15‑FITC, CD66b-PE, as well as CD45‑VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for
StraightFrom
®
Whole Blood CD66b MicroBeads
, human

Overview

StraightFrom
®
Whole Blood CD66b MicroBeads were developed for rapid positive selection of CD66b
+
cells directly from human whole blood. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis.

Detailed product information

Background information

StraightFrom
®
Whole Blood CD66b MicroBeads are developed for the positive selection of CD66b
+
cells directly from anticoagulated whole blood by using the autoMACS
®
Pro Separator, the MultiMACS™ Cell24 Separator Plus, or the Whole Blood Column Kit. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis. CD66b is a 95–100 kDa glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. It is expressed on neutrophils and eosinophils but not on basophils and lymphocytes.

Detailed separation procedure

Anticoagulated whole blood samples of 0.25–15 mL are labeled with StraightFrom
®
Whole Blood CD66b MicroBeads to be subsequently separated with the autoMACS
®
Pro Separator, the MultiMACS™ Cell24 Separator Plus, or using the Whole Blood Column Kit.

Columns

autoMACS
®
Columns or Whole Blood Columns.

Resources for
StraightFrom
®
Whole Blood CD66b MicroBeads
, human

Video

How to isolate immune cells directly from blood

Follow this fast and easy protocol for magnetic isolation of immune cells directly from blood or blood products without the need for density gradient centrifugation.

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®
Whole Blood CD66b MicroBeads
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