Clone:
REA530
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC
Alternative names:
PLK-1, STPK13

Specifications for PLK1 pT210 Antibody, anti-human, REAfinity™

Overview

Clone REA530 recognizes the human polo-like kinase (PLK1) antigen phosphorylated at threonine 210 (pT210). PLK1 is a key regulator of several important cell-cycle–associated processes, such as centrosome maturation, spindle assembly, sister chromatid cohesion, cytokinesis, and recovery from a DNA-damage–induced arrest. Expression of PLK1 is highly cell cycle regulated; its expression is first induced in G2 and peaks during the early stages of mitosis. PLK1 has been described to be recruited to the centrosomes in G2, as well as to spindle poles and kinetochores during mitosis. Activation of PLK1 is dependent on phosphorylation of a conserved threonine residue (T210) in the T-loop of its kinase domain. Phosphorylation of this residue starts in G2, when PLK1 activity gradually increases until it reaches its full activity when cells enter mitosis. The initial phosphorylation of T210 in G2 is mediated by the kinase Aurora-A, in concert with its cofactor Bora. Since PLK1 is highly expressed in several carcinomas, and expression is inversely correlated with the survival rate of patients in non-small cell lung, head and neck, and esophageal cancer, PLK1 is recognized as a valid prognostic marker.
Additional information: Clone REA530 displays negligible binding to Fc receptors.

Alternative names

PLK-1, STPK13

Detailed product information

Technical specifications

CloneREA530
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenPLK1 pT210
Alternative names of antigenPLK-1, STPK13
Molecular mass of antigen [kDa]68
Distribution of antigenother
Entrez Gene ID5347
RRIDAB_2653258, AB_2653259, AB_2653260, AB_2653257

References for PLK1 pT210 Antibody, anti-human, REAfinity™

Publications

  1. Bruinsma, W. et al. (2014) Bora and Aurora-A continue to activate Plk1 in mitosis. J. Cell. Sci. 127(4): 801-811
  2. Hamanaka, R. et al. (1994) Cloning and characterization of human and murine homologues of the Drosophila polo serine-threonine kinase. Cell Growth Differ. 5(3): 249-257
  3. Kakeno, M. et al. (2014) Plk1 phosphorylates CLIP-170 and regulates its binding to microtubules for chromosome alignment. Cell Struct. Funct. 39(1): 45-59

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