The MACSxpress
®
Whole Blood NK Cell Isolation Kit was developed for the isolation of untouched natural killer (NK) cells from anticoagulated whole blood without density gradient centrifugation.

Data and images for
MACSxpress
®
Whole Blood NK Cell Isolation Kit
, human

Figures

Figure 1

Untouched NK cells were isolated from 8 mL of human EDTA-anticoagulated whole blood using the MACSxpress
®
Whole Blood NK Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45-VioBlue, CD3-FITC, and CD56-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer 10.
Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.
Before separation
After separation
View details

Figure 1

Untouched NK cells were isolated from 8 mL of human EDTA-anticoagulated whole blood using the MACSxpress
®
Whole Blood NK Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45-VioBlue, CD3-FITC, and CD56-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer 10.
Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.
View details

Figure 1

Untouched NK cells were isolated from 8 mL of human EDTA-anticoagulated whole blood using the MACSxpress
®
Whole Blood NK Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45-VioBlue, CD3-FITC, and CD56-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer 10.
Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.

Specifications for
MACSxpress
®
Whole Blood NK Cell Isolation Kit
, human

Overview

The MACSxpress
®
Whole Blood NK Cell Isolation Kit was developed for the isolation of untouched natural killer (NK) cells from anticoagulated whole blood without density gradient centrifugation.

Detailed product information

Detailed separation procedure

With the new MACSxpress
®
Technology, untouched human leukocyte subsets can be isolated from up to 30 mL of anticoagulated whole blood. Non-target cells are removed by immunomagnetic depletion using MACSxpress Beads. Simultaneously, erythrocytes are sedimented, yielding untouched target cells of high purity.

Downstream applications

NK cells isolated with the MACSxpress
®
Whole Blood NK Cell Isolation Kit can be used, e.g., for phenotyping, NK cell expansion or cytotoxicity assays.

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MACSxpress
®
Whole Blood NK Cell Isolation Kit
, human

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