Our MACSPlex Mix products allow you to combine individual analytes within one application category according to your needs. This option is available for the MACSPlex Cytokine Kit, human, MACSPlex Cytotoxic T/NK Cell Kit, human, and the MACSPlex Cytokine Kit, mouse.
To put together your MACSPlex Mix, you need the following components:
Because each single reagent has a specific fluorescence property, the combination of analytes is only possible within one defined application category. Cytokine Kit, human, MACSPlex Cytotoxic T/NK Cell Kit, human and the MACSPlex Cytokine Kit, mouse cannot be mixed within a single experiment.
Overview of combinable reagents and necessary software versions to run Express Modes
|Application category||Available target analytes||Standard||Basic Kit|
|Human Cytokine 12||IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12, IL-17A, GM-CSF, IFN-α, IFN-γ, TNF-α||MACSPlex Cytokine 12 Standard, human (130-106-197)||MACSPlex Cytokine Basic Kit, human and mouse |
|Mouse Cytokine 10||IL-2, IL-4, IL-5, IL-10, IL-12, IL-17A, IL-23, GM-CSF, IFN-γ, TNF-α||MACSPlex Cytokine 10 Standard, mouse (130-106-198)|
Cytotoxic T/NK Cell
|IL-2, IL-4, IL-6, IL-10, IL17A, IL-21, GM-CSF, Granzyme B, IFN-γ, MCP-1, TNF-α, Perforin ||MACSPlex Mix Cytotoxic Standard |
|MACSPlex Mix Cytotoxic Basic Kit (130-125-767)|
For MACSPlex assays a flow cytometer with a blue (e.g. 488 nm) and a red (e.g. 635 nm) laser with the capability to detect FITC, PE, and APC is necessary.
ExpressModes are compatible with the MACSQuant Analyzer 10 and MACSQuant X instruments and with the MACSQuantify™ Software already installed on your MACSQuant Analyzer. For the required software version, please refer to the table above.
For all users running MACSPlex assays on a different flow cytometer, subsequent analysis can be performed on your computer. For this, you can use our Flowlogic™ Software in combination with the Beadlogic™ Add-on specifically developed to analyze multiplex assays.
Additionally, third-party software can be used for analysis.