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PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Subsequently, cells were analyzed for IL-13 secretion as well as CD154 expression using the IL-13 Secretion Assay - Detection Kit, and CD154-APC. The plots show IL-13 secretion and CD154 expression by viable CD4+
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