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PBMCs were stimulated with PMA and Ionomycin or left untreated. Cells were stained according to secretion of GM-CSF using the GM-CSF Secretion Assay - Detection Kit (PE). The cells were counterstained with CD4-APC. B cells, monocytes and dead cells were excluded according to CD20-PerCP-Vio700, CD14-PerCP-Vio700 and PI-staining respectively.
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