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Splenocytes from BALB/c mice were first labeled with anti-mouse CD268 (isotype rat IgG1) and then stained with Anti-IgG1 antibodies or with the corresponding isotype control antibodies (left image), as well as with CD19 antibodies. Flow cytometry was performed with the MACSQuant®
Analyzer. Lymphocytes were pre-gated for the analysis. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or DAPI fluorescence, as in the case of tandem-conjugates.
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