REAlease® CD304 (BDCA-4) Complexes, human

REAlease
®
CD304 (BDCA-4) Complexes
, human

Clone: REAL222 | Dilution: 1:50
Clone REAL222 is an antibody fragment derived from the full CD304 (BDCA-4) antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL222 recognizes the human CD304 (BDCA-4) antigen. Like CD303 (BDCA-2), the CD304 (BDCA-4) antigen is specifically expressed on human plasmacytoid dendritic cells in blood and bone marrow. Unlike CD303 (BDCA-2), expression of CD304 (BDCA-4) is up-regulated on plasmacytoid dendritic cells and induced on myeloid cells upon culturing. In inflamed tonsils, CD304 expression, apart from plasmacytoid dendritic cells, is also detected primarily on follicular B helper memory T cells. The CD304 (BDCA-4) is identical to neuropilin-1 (NP-1), a neuronal receptor, which is known to be expressed on numerous nonhematopoietic cell types, including neurons, endothelial, and tumor cells. CD1a
+
dendritic cells generated
ex vivo
from monocytes or hematopoietic precursor cells are CD304
+
but CD303 (BDCA-2).
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

NRP1, BDCA-4, NP1, Nrp, VEGF165R, Neuropilin-1

Technical specifications

  • Antigen: CD304 (BDCA-4)
  • Clone: REAL222
  • Isotype control: REAL Control
  • Alternative names of antigen: NRP1, BDCA-4, NP1, Nrp, VEGF165R, Neuropilin-1
  • Distribution of antigen: dendritic cells, bone marrow, B cells, T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC
  • Selected references

    1. Grabbe, S. et al. (2000) Dendritic cells: multi-lineal and multi-functional. Immunol. Today 21: 431-433
    2. Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J. Immunol. 165: 6037-6046
    3. Dzionek, A. et al. (2001) BDCA-2, a novel plasmacytoid dendritic cell–specific type II C-type lectin, mediates antigen capture and is a potent inhibitor of interferon α/β induction. J. Exp. Med. 194: 1823-1834
    4. Dzionek, A. et al. (2002) Plasmacytoid dendritic cells: from specific surface markers to specific cellular functions. Hum. Immunol. 63: 1133-1148
    5. Jongbloed, S. L. et al. (2010)
      Human CD141
      +
      (BDCA-3)
      +
      dendritic cells (DCs) represent a unique myeloid DC subset that cross-presents necrotic cell antigens.
      J. Exp. Med. 207(6): 1247-1260
    6. Mittag, D. et al. (2011) Human dendritic cell subsets from spleen and blood are similar in phenotype and function but modified by donor health status. J. Immunol. 186(11): 6207-6217
    7. Moschella, F. et al. (2013) Cyclophosphamide induces a type I interferon-associated sterile inflammatory response signature in cancer patients' blood cells: implications for cancer chemoimmunotherapy. Clin. Cancer Res. 19(15): 4249-4261
    8. Jardine, L. et al. (2013) Rapid detection of dendritic cell and monocyte disorders using CD4 as a lineage marker of the human peripheral blood antigen-presenting cell compartment. Front Immunol 4: 495
Product options: 6
for 30
tests
(1)
CHF  145.00
for 100
tests
(1)
CHF  350.00
for 30
tests
(1)
CHF  145.00
for 100
tests
(1)
CHF  350.00
for 100
tests
(1)
CHF  410.00
for 100
tests
(1)
CHF  410.00

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