CD317 (PDCA-1) antibodies – functional grade, mouse

Technical specifications

• Antigen: CD317 (PDCA-1)
• Clone: JF05-1C2.4.1
• Isotype: rat IgG2b
• Alternative names of antigen: BST2, Bst-2, C87040, CD317, DAMP-1, GREG
• Entrez Gene ID: 69550
• Molecular mass of antigen [kDa]: 17
• Distribution of antigen: dendritic cells
• Endotoxin level: Endotoxin levels have been tested and do not exceed 0.01 ng/µg of protein.
• Product format: Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: pure

• Figure 1

  Mouse PDCs were specifically depleted in BALB/c mice by i.p. injection of 500 µg CD317 (PDCA-1) pure – functional grade. The frequency of CD317 (PDCA-1) + PDCs was analyzed in spleen 24 hours after injection of the antibody or diluent only (control mice) by staining with CD317 (PDCA-1) antibodies. To exclude blocking of CD317 (PDCA-1) staining by the in vivo applied CD317 (PDCA-1) antibody, cells were additionally stained with Anti-Ly-6C and CD45R (B220) antibodies. Note that the frequency of PDCs decreases in mice which received the CD317 (PDCA-1) antibody (A) to about one fifth compared to control mice (B), independent of whether it is analyzed based on CD317 (PDCA-1) staining or on Anti-Ly-6C and CD45R (B220) staining. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

  A) Anti-mPDCA-1 antibody

  B) Control

• Selected references

  1. Fischer et al. (2004) MACS&more 8(1): 13-15
  2. Krug, A. et al. (2004) TLR9-dependent recognition of MCMV by IPC and DC generates coordinated cytokine responses that activate antiviral NK cell function. Immunity 21: 107-119
  3. Yoneyama, H. et al. (2005) Plasmacytoid DCs help lymph node DCs to induce anti-HSV CTLs. J. Exp. Med. 202(3): 425-435
  4. Shen, H. and Iwasaki, A. (2006) A crucial role for plasmacytoid dendritic cells in antiviral protection by CpG ODN-based vaginal microbicide. J. Clin. Invest. 116: 2237-2243
  5. Kuwajima, S. et al. (2006) Interleukin 15-dependent crosstalk between conventional and plasmacytoid dendritic cells is essential for CpG-induced immune activation. Nat. Immunol. 7: 740-746