CD317 (PDCA-1) antibodies – functional grade, mouse

CD317 (PDCA-1) antibodies – functional grade, mouse

Clone: JF05-1C2.4.1
Clone JF05-1C2.4.1 recognizes the mouse CD317 antigen, also known as plasmacytoid dendritic cell antigen-1 (PDCA-1). CD317 is specifically expressed on mouse PDCs, a subset of CD11c
+
dendritic cells detected at low frequency in all lymphoid tissues, peripheral blood, and some non-lymphoid tissues.
In mouse spleen, bone marrow, and lymph nodes, PDCA-1 is exclusively expressed on cells which are CD11c
low
, Ly-6C
high
, CD45R (B220)
+
, MHC class II
low
, CD8a
low/–
, CD80
low/–
, CD86
–
, CD40
–
, CD11b
–
, CD90
–
, CD49b (DX5)
–
, CD3
–
, CD19
–
, i.e. on cells with the phenotype of mouse PDCs. Multi-color fluorescent staining of spleen cells clearly revealed that all CD11c
low
Ly-6C
high
CD45R (B220)
+
PDCs are PDCA-1
+
and that PDCA-1 expression is restricted to PDCs.

Applications

The CD317 (PDCA-1) pure – functional grade antibody is suited for
in vivo
depletion of PDCs for studies on their functional properties in experimental mouse models of infection and autoimmunity.

Alternative names

BST2, Bst-2, C87040, CD317, DAMP-1, GREG

Technical specifications

  • Antigen: CD317 (PDCA-1)
  • Clone: JF05-1C2.4.1
  • Isotype: rat IgG2b
  • Alternative names of antigen: BST2, Bst-2, C87040, CD317, DAMP-1, GREG
  • Entrez Gene ID: 69550
  • Molecular mass of antigen [kDa]: 17
  • Distribution of antigen: dendritic cells
  • Endotoxin level: Endotoxin levels have been tested and do not exceed 0.01 ng/µg of protein.
  • Product format: Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: pure

  • Figure 1

    Mouse PDCs were specifically depleted in BALB/c mice by i.p. injection of 500 µg CD317 (PDCA-1) pure – functional grade. The frequency of CD317 (PDCA-1)
    +
    PDCs was analyzed in spleen 24 hours after injection of the antibody or diluent only (control mice) by staining with CD317 (PDCA-1) antibodies. To exclude blocking of CD317 (PDCA-1) staining by the
    in vivo
    applied CD317 (PDCA-1) antibody, cells were additionally stained with Anti-Ly-6C and CD45R (B220) antibodies. Note that the frequency of PDCs decreases in mice which received the CD317 (PDCA-1) antibody (A) to about one fifth compared to control mice (B), independent of whether it is analyzed based on CD317 (PDCA-1) staining or on Anti-Ly-6C and CD45R (B220) staining. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
    A) Anti-mPDCA-1 antibody
    B) Control

  • Selected references

    1. Fischer et al. (2004) MACS&more 8(1): 13-15
    2. Krug, A. et al. (2004) TLR9-dependent recognition of MCMV by IPC and DC generates coordinated cytokine responses that activate antiviral NK cell function. Immunity 21: 107-119
    3. Yoneyama, H. et al. (2005) Plasmacytoid DCs help lymph node DCs to induce anti-HSV CTLs. J. Exp. Med. 202(3): 425-435
    4. Shen, H. and Iwasaki, A. (2006) A crucial role for plasmacytoid dendritic cells in antiviral protection by CpG ODN-based vaginal microbicide. J. Clin. Invest. 116: 2237-2243
    5. Kuwajima, S. et al. (2006) Interleukin 15-dependent crosstalk between conventional and plasmacytoid dendritic cells is essential for CpG-induced immune activation. Nat. Immunol. 7: 740-746
Product options: 2
130-091-978

CD317 (PDCA-1) pure - functional grade, mouse

1 mg in 0.5 mL
CHF  805.00
130-092-550

CD317 (PDCA-1) pure - functional grade, mouse

8 mg in 4 mL
CHF  3'340.00

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