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A single-cell suspension was prepared using the Epidermis Dissociation Kit. Cells were stained with CD207 (Langerin) antibodies conjugated to FITC (A), PE (B), APC (C), VioBlue (D), or PE-Vio770 (E) as well as with Anti-MHC Class II antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cells labeled with CD207 (Langerin)- Biotin (F) were stained with Anti-Biotin-PE in addition. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of CD207(Langerin)-PE-Vio770.
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