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Bone marrow cells from C57BL/6 mice were stained with CD200R3 antibodies as well as with CD49b (DX5) antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. The specificity of the conjugated antibodies was confirmed by blocking the binding to the ligand, using pure unconjugated antibodies (left images). Cell debris and dead cells were excluded from the analysis based on scatter signals and either propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.
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