CD135 (FLT3) antibodies, human

CD135 (FLT3) antibodies, human

Clone: BV10A4H2 | Dilution: 1:11
Clone BV10A4H2 recognizes the human CD135 antigen, a single-pass type I membrane protein also known as Fms-like tyrosine kinase receptor 3 (FLT3) or fetal liver kinase-2 (FLK-2). CD135 belongs to the same family as FMS, KIT, and the two genes encoding PDGFRα and β. CD135 is normally expressed by hematopoietic stem and progenitor cells and expression is lost as hematopoietic cells differentiate. Although CD135 expression is usually lost upon HSC differentiation, dendritic cells are an exception, as mature DCs display persistent FLT3 expression. CD135 is also expressed on malignant hematopoietic cells including AML, ALL, and CML-BC. All hematopoietic cells develop from CD135 negative hematopoietic stem cells through CD135 positive progenitor cells. In synergy with other growth factors like G-CSF, GM-CSF, SCF, and IL-3, ligation of CD135 with FLT3 ligand promotes the differentiation, proliferation, and survival of hematopoietic progenitor cells and of dendritic cells. As a result early B cell lineage differentiation as well as expansion of monocytes and immature dendritic cells are stimulated.

Alternative names

FLT3, FLK-2, STK-1

Technical specifications

  • Antigen: CD135 (FLT3)
  • Clone: BV10A4H2
  • Isotype: mouse IgG1κ
  • Isotype control: Mouse IgG1 – isotype control antibodies
  • Alternative names of antigen: FLT3, FLK-2, STK-1
  • Entrez Gene ID: 2322
  • Molecular mass of antigen [kDa]: 110
  • Distribution of antigen: hematopoietic stem cells, dendritic cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC, PE-Vio770, Biotin
  • Selected references

    1. Small, D. et al. (1994)
      STK-1, the human homolog of Flk-2/Flt-3, is selectively expressed in CD34
      +
      human bone marrow cells and is involved in the proliferation of early progenitor/stem cells.
      Proc. Natl. Acad. Sci. U.S.A. 91(2): 459-463
    2. Leung, A. Y. et al. (2013) FLT3 inhibition: a moving and evolving target in acute myeloid leukaemia. Leukemia 27(2): 260-268
    3. Rosnet, O. et al. (1996) Human FLT3/FLK2 receptor tyrosine kinase is expressed at the surface of normal and malignant hematopoietic cells. Leukemia 10(2): 238-248
Product options: 8
for 30
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CHF  120.00
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CHF  290.00
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CHF  120.00
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tests
(1)
CHF  290.00
for 30
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(1)
CHF  130.00
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tests
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CHF  315.00
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(1)
CHF  100.00
for 100
tests
(1)
CHF  245.00

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