Anti-SLP-76 antibodies, human

Anti-SLP-76 antibodies, human

Clone: REA427 | Dilution: 1:11
Clone REA427 recognizes the human SH2 domain-containing leukocyte protein of 76 kDa (SLP-76) regardless of phosphorylation status. SLP-76 is a signal-transducing tyrosine phosphoprotein also known as lymphocyte cytosolic protein 2 (LCP2). It is involved in T cell antigen receptor–mediated signaling and is highly expressed in spleen, thymus, and peripheral blood leukocytes. The adapter molecule SLP-76 plays a central roles in T cell activation by recruiting enzymes and other adapters into multiprotein complexes that coordinate highly regulated signal transduction pathways. TCR stimulation facilitates phosphorylation of the TCRζ chains by the Src family kinase Lck, which allows for the recruitment and activation of the protein tyrosine kinase Zap-70. Phosphorylation of the essential adapter protein SLP-76 by Zap-70 creates docking sites for SH2 domain-containing adapter and effector proteins. The Grb2-related adapter, Gads, recruits the adapter SLP-76. SLP-76 recruits other signaling proteins, thereby inducing the assembly of multiprotein complexes.
Additional information: Clone REA427 displays negligible binding to Fc receptors.

Alternative names

LCP2, SLP76

Technical specifications

  • Antigen: SLP-76
  • Clone: REA427
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (I) antibodies
  • Alternative names of antigen: LCP2, SLP76
  • Entrez Gene ID: 3937
  • Molecular mass of antigen [kDa]: 60
  • Distribution of antigen: spleen, leukocytes, other
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC
  • Selected references

    1. Jackman, J. K. et al. (1995) Molecular cloning of SLP-76, a 76-kDa tyrosine phosphoprotein associated with Grb2 in T cells. J. Biol. Chem. 270(13): 7029-7032
    2. Sela, M. et al. (2011) Sequential phosphorylation of SLP-76 at tyrosine 173 is required for activation of T and mast cells. EMBO J. 30(15): 3160-3172
    3. Coussens, N. P. et al. (2013) Multipoint binding of the SLP-76 SH2 domain to ADAP is critical for oligomerization of SLP-76 signaling complexes in stimulated T cells. Mol. Cell. Biol. 33(21): 4140-4151
Product options: 6
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CHF  120.00
for 100
tests
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CHF  290.00
for 30
tests
(1)
CHF  120.00
for 100
tests
(1)
CHF  290.00

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