Anti-PSA-NCAM antibodies, human, mouse, rat

Anti-PSA-NCAM antibodies, human, mouse, rat

Clone: 2-2B
The Anti-PSA-NCAM antibody recognizes an epitope on human, mouse, and rat polysialic acid (PSA) which, in vertebrates, is found linked to the extracellular domain of the neural cell adhesion molecule (NCAM, CD56). PSA-NCAM, the highly polysialated form of NCAM, is predominantly expressed in embryonic and neonatal neural tissue. In adult mammalian brain PSA-NCAM expression is restricted mainly to areas that retain neurogenic potential, such as the subventricular zone (SVZ) and the dentate gyrus of the hippocampus. PSA-NCAM is a marker for immature neuronal-committed progenitors that are permanently generated in the SVZ and migrate along a well-defined pathway, the rostral migratory stream, into the olfactory bulb where they differentiate into GABAergic and dopaminergic interneurons. Antibodies against PSA-NCAM have also been used to immunomagnetically isolate neuronal progenitors from postnatal mice and rat forebrain.

Alternative names

NCAM1, CD56, MSK39, NCAM, E-NCAM, NCAM-1, NCAM-C

Technical specifications

  • Antigen: PSA-NCAM
  • Clone: 2-2B
  • Isotype: mouse IgM
  • Isotype control: Mouse IgM – isotype control antibodies
  • Alternative names of antigen: NCAM1, CD56, MSK39, NCAM, E-NCAM, NCAM-1, NCAM-C
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: pure
  • Selected references

    1. Jungblut, M. et al. (2012) Isolation and characterization of living primary astroglial cells using the new GLAST-specific monoclonal antibody ACSA-1. Glia 60(6): 894-907
    2. G. Kantzer, C. et al. (2017) Anti-ACSA-2 defines a novel monoclonal antibody for prospective isolation of living neonatal and adult astrocytes. Glia 65(6): 990-1004
    3. Batiuk, M. Y. et al. (2017) An immunoaffinity-based method for isolating ultrapure adult astrocytes based on ATP1B2 targeting by the ACSA-2 antibody. J. Biol. Chem. 292(21): 8874-8891
    4. Rougon, G. and Marshak, D. R. (1986) Structural and immunological characterization of the amino-terminal domain of mammalian neural cell adhesion molecules. J. Biol. Chem. 261: 3396-3401
    5. Kiss, J. Z. and Muller, D. (2001) Contribution of the neural cell adhesion molecule to neuronal and synaptic plasticity. Rev. Neurosci. 12: 297-310
    6. Doetsch, F. et al. (1997) Cellular composition and three-dimensional organization of the subventricular germinal zone in the adult mammalian brain. J. Neurosci. 17: 5046-5061
    7. Seki, T. (2002) Expression patterns of immature neuronal markers PSA-NCAM, CRMP-4 and NeuroD in the hippocampus of young adult and aged rodents. J. Neurosci. Res. 70: 327-334
    8. Pennartz, S. et al. (2004) Purification of neuronal precursors from the adult mouse brain: comprehensive gene expression analysis provides new insights into the control of cell migration, differentiation, and homeostasis. Mol. Cell. Neurosci. 25: 692-706
    9. Seidenfaden, R. et al. (2006) Glial conversion of SVZ-derived committed neuronal precursors after ectopic grafting into the adult brain. Mol. Cell. Neurosci. 32: 187-198
    10. Seidenfaden et al. (2006) MACS&more 10(1): 4-6
    11. Marmur, R. et al. (1998) Differentiation of oligodendroglial progenitors derived from cortical multipotent cells requires extrinsic signals including activation of gp130/LIFbeta receptors. J. Neurosci. 18: 9800-9811
    12. Metsuyanim, S. et al. (2009) Expression of stem cell markers in the human fetal kidney. PLoS One 4(68): e6709
  • gentleMACS Protocols

  • Special protocols

Product options: 1
100 µg in 1 mL
CHF  528.00