Anti-IL-5 antibodies, human

Anti-IL-5 antibodies, human

Clone: JES1-39D10 | Dilution: 1:10
Interleukin 5 (IL-5) is pre-dominantly secreted by CD4
+
T cells. It is involved in a range of allergic reactions and mediates immune reactions against parasites. IL-5 also acts on other cell types such as B cells.
The Anti-IL-5 antibody has been designed for intracellular staining of IL-5–producing cells. Cells can be stimulated for IL-5-production, for example, by polyclonal stimulation with mitogens. For induction of IL-5 production by antigen-specific T cells, cells are restimulated with respective antigen. IL-5 can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, IL-5–producing cells can be stained intracellularly with Anti-IL-5 antibodies. Staining of surface markers allows simultaneous flow cytometric analysis of subsets and activation status of the IL-5–producing cells. Magnetically enriched cells can be stained intracellularly for IL-5 production directly on the MACS
®
Column. This procedure ensures higher sensitivity of detection and minimizes loss of cells during washing procedures. The protocol is useful for cytokine analysis of rare cells, for example, CD4
+
T cells in HIV patients, or other cell sources than peripheral blood mononuclear cells (PBMCs), e.g., bronchoalveolar lavages or synovial fluids.

Alternative names

EDF, TRF

Technical specifications

  • Antigen: IL-5
  • Clone: JES1-39D10
  • Isotype: rat IgG2aκ
  • Isotype control: Rat IgG2a – isotype control antibodies
  • Alternative names of antigen: EDF, TRF
  • Entrez Gene ID: 3567
  • Molecular mass of antigen [kDa]: 13
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    )
    ,
    cynomolgus monkey (
    Macaca fascicularis
    )
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: APC, Vio515, Vio667, PE
Product options: 6
for 100
tests
(1)
CHF  290.00
for 100
tests
(1)
CHF  290.00
for 30
tests
CHF  125.00
for 100
tests
CHF  305.00
for 30
tests
CHF  125.00
for 100
tests
CHF  305.00

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