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Mouse RAW264.7 cells, either left unstimulated (left peak) or stimulated with IFN-γ and LPS for 19 hours, followed by an incubation with brefeldin A for 5 hours, were fixed, permeabilized and stained with Anti-IL-27 p28 antibodies. Flow cytometry was performed with the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
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