Anti-IL-17A antibodies, human

Anti-IL-17A antibodies, human

Clone: CZ8-23G1 | Dilution: 1:10
Clone CZ8-23G1 detects human interleukin 17 (IL-17). Interleukin 17A (CTLA8), a member of the IL-17 family (IL-17A–F), is a disulfide-linked homodimeric glycoprotein. Human IL-17A consists of 155 amino acids with a molecular weight of around 35 kDa. IL-17A is produced by CD4
+
T helper (Tʜ) cells, a third T cell subset termed Tʜ17, which secrete also cytokines such as IL-17F and IL-22 and express the NK cell marker CD161. IL-17A secretion has also been described for other cell types, such as CD8
+
memory T cells. Furthermore, intracellular IL-17A has also been detected in eosinophils, neutrophils, and blood monocytes. Emerging data about Tʜ17 cells suggest that these cells are involved in the recruitment of neutrophils to control early stages of infections to a number of pathogens, such as extracellular bacteria and fungi. IL-17A and Tʜ17 cells have been shown to play an important role in immune-mediated diseases, such as rheumatoid arthritis, psoriasis, multiple sclerosis, asthma, inflammatory bowel diseases, and other immune-mediated inflammatory conditions.
Depending on the cytokine milieu present at time of the initial engagement, CD4
+
naive T cells can differentiate into various subsets (Tʜ1, Tʜ2, and Tʜ17). For the differentiation into Tʜ17 cells several cytokines have been described, including TGF-β, IL-1β, IL-6, IL-21, and IL-23. RORγt was identified as the master regulator gene for Tʜ17 cells.

Alternative names

CTLA-8, IL-17

Technical specifications

  • Antigen: IL-17A
  • Clone: CZ8-23G1
  • Isotype: mouse IgG1κ
  • Isotype control: Mouse IgG1 – isotype control antibodies
  • Alternative names of antigen: CTLA-8, IL-17
  • Entrez Gene ID: 3605
  • Molecular mass of antigen [kDa]: 15
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    )
    ,
    cynomolgus monkey (
    Macaca fascicularis
    )
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: APC, VioBlue, PE-Vio615, APC-Vio770

  • Figure 1

    Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left images) or stimulated with CytoStim for six hours. After two hours, brefeldin A was added for stimulated and unstimulated cells. The cells were fixed, permeabilized, and intracellularly stained with Anti-IL-17A antibodies as well as with CD4 antibodies and CD154 antibodies. Cells were then analyzed by flow cytometry using the MACSQuant
    ®
    Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. CD4
    +
    lymphocets were pre-gated for the analysis. Cell debris were excluded from the analysis based on scatter signals.

  • Figure 2

    PBMCs from cynomolgus monkey were incubated with CytoStim for 6 hours or left untreated. After two hours, brefeldin A was added. The cells were fixed, permeabilized, and intracellularly stained with Anti-IL-17A-PE and CD154-APC. Cells were analyzed by flow cytometry using the MACSQuant
    ®
    Analyzer.
    CytoStim-stimulated PBMCs
    Unstimulated control

  • Figure 3

    PBMCs from rhesus monkey were incubated with CytoStim for 6 hours or left untreated. After two hours, brefeldin A was added. The cells were fixed, permeabilized, and intracellularly stained with Anti-IL-17A-PE and CD154-APC. Cells were analyzed by flow cytometry using the MACSQuant
    ®
    Analyzer.
    CytoStim-stimulated PBMCs
    Unstimulated control

  • Selected references

    1. Yao, Z. et al. (1995) Human IL-17: a novel cytokine derived from T cells. J. Immunol. 155: 5483-5486
    2. Cosmi, L. et al. (2008)
      Human interleukin 17–producing cells originate from a CD161
      +
      CD4
      +
      T cell precursor.
      J. Exp. Med. 205: 1903-1916
    3. Kolls, J. K. and Linden, A. (2004) Interleukin-17 family members and inflammation. Immunity 21: 467-476
    4. Tesmer, L. A. et al. (2008) Tʜ17 cells in human disease. Immunol. Rev. 223: 87-113
    5. Yang, L. et al. (2008) IL-21 and TGF-beta are required for differentiation of human Tʜ17 cells. Nature 454: 350-352
    6. Manel, H. et al. (2008) The differentiation of human Tʜ-17 cells requires transforming growth factor-β and induction of the nuclear receptor RORγt. Nat. Immunol. 9: 641-649
    7. Volpe, E. et al. (2008) A critical function for transforming growth factor-β, interleukin 23 and proinflammatory cytokines in driving and modulating human Tʜ-17 responses. Nat. Immunol. 9: 650-657
    8. Ivanov, I. I. et al. (2006)
      The orphan nuclear receptor RORγt directs the differentiation program of proinflammatory IL-17
      +
      T helper cells.
      Cell 126: 1121-1133
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