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Splenocytes from BALB/c mice, either left unstimulated (left images) or stimulated with CD3/CD28 antibodies and1 µg/mL brefeldin A, were fixed and permeabilized. Cells were then stained with Anti-IFN-γ antibodies as well as with CD154 antibodies and analyzed by flow cytometry using the MACSQuant®
cells were pre-gated for the analysis. Cell debris were excluded from the analysis based on scatter signals.
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