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Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left images) or stimulated with PMA/ionomycin for 6 hours. After 2 hours, brefeldin A was added. Cells were then fixed, permeabilized, and intracellularly stained with Anti-GMCSF antibodies and CD4 antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Gating was performed according to forward and side scatter properties of the cells.
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