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Human peripheral blood cells after erythrocyte lysis were stained with Anti-C3a Receptor antibodies or with the corresponding isotype control antibodies (left image). The FcR Blocking Reagent has been used to avoid Fc receptor-mediated antibody labeling. Flow cytometry was performed with the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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