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Thymocytes from C57BL/6 mice were fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with Anti-c-Rel antibodies or with the corresponding REA Control antibodies (left peak) and analyzed by flow cytometry using the MACSQuant®
cells were pre-gated for the analysis. Cell debris were excluded from the analysis based on scatter signals.
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