StraightFrom® Whole Blood MicroBeads

Cell isolation directly from whole blood

  • Isolation of highly pure cells straight from whole blood in 30 minutes
  • No density gradient centrifugation, no erythrocyte lysis
  • Ready-to-use kits and fast protocols for manual or automated cell isolations

StraightFrom® Whole Blood MicroBeads enable magnetic cell separation directly from whole blood. Leukocyte subsets such as CD4+, CD8+, CD14+, CD19+, or CD56+ cells can be purified using either cell separation instruments or manual separators. Pre-enrichment of white blood cells, e.g. by density gradient centrifugation, is not required. The purified cells are well-suited for further flow cytometric analyses, molecular biology applications, and functional studies.

Whole blood as starting material

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For numerous applications, leukocyte subsets need to be isolated from anticoagulated blood samples. Whole blood donations represent a readily available and versatile source material with well-defined cell numbers that come in any desired volume. StraightFrom® Whole Blood MicroBeads were designed to optimally fit this flexibility.

Manual cell separation

Manual isolation of pure cells directly from whole blood.
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StraightFrom Whole Blood MicroBead Kits are suited for the separation of leukocyte subsets directly from anticoagulated human whole blood.

The MicroBeads are added directly to the whole blood sample without any prior cell counting or PBMC isolation. Leukocytes can thereby be isolated straightforward within minutes without the need for density gradient centrifugation.

Find a product list of all StraightFrom Whole Blood MicroBead Kits at the end of this page.

Automated cell isolation

Automated isolation straight from whole blood using the autoMACS Pro Separator.
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In contrast to manual separation using MACS Separators, cell separation from whole blood can also be performed using the autoMACS® Pro Separator for walk-away automation, or the MultiMACS Cell24 Separator Plus for semi-automated separation. Both instruments allow processing of high sample volumes or numbers and provide convenient and easy sample handling. This improves reliability and reproducibility in day-to-day cell separation.

For large-scale cell separations, the MultiMACS X is the instrument of choice.

Data

Separation of CD3+ cells  using StraightFrom® Whole Blood CD3 MicroBeads

CD3+ cells can be isolated straight from a whole blood sample using the StraightFrom® Whole Blood CD3 MicroBeads, human and the MultiMACS™ Cell24 Separator Plus.

Before separation
After separation

Separation of a whole blood sample using the StraightFrom® Whole Blood CD3 MicroBeads, human and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns without washing step after labeling. Cells were fluorescently stained with CD3-PE, CD14‑APC, as well as CD45-VioBlue®and analyzed by flow cytometry using the MACSQuant® Analyzer.

Videos

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Video

How to isolate immune cells directly from blood

Follow this fast and easy protocol for magnetic isolation of immune cells directly from blood or blood products using the MultiMACS™ Cell24 Separator Plus.

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Webinar

Immune cell isolation without density gradient centrifugation

Learn from this webinar how to isolate immune cells straight from blood or blood products without density gradient centrifugation.

 

 

Resources

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Brochure

StraightFrom MicroBeads - Cell isolation directly from blood products

Download the brochure to get all the details on our StraightFrom MicroBead Technology.

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Scientific poster

Isolation of functional cells straight from buffy coat, LRSC, and Leukopak®

Maike Schlemminger, Silke Weber-Lohmann, Martin Kolnik*, Zhong Yu, Susanne Höher-Peters, and Gregor Winkels

Miltenyi Biotec, Bergisch Gladbach, Germany

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