StraightFrom® Whole Blood CD4 MicroBeads, human

StraightFrom
®
Whole Blood CD4 MicroBeads
, human

StraightFrom
®
Whole Blood CD4 MicroBeads were developed for rapid automated positive selection of CD4
+
cells directly from whole blood or bone marrow. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis.

Background information

StraightFrom
®
Whole Blood CD4 MicroBeads recognize about 65% of all peripheral blood T cells. The CD4 antigen is expressed on T helper cells and at a lower level on monocytes and dendritic cells. The CD4 antigen is an accessory molecule in the recognition of MHC class II/peptide complexes by the TCR heterodimer on CD4
+
T helper cells.

Detailed separation procedure

Anticoagulated cell samples of 0.25–15 mL are labeled with StraightFrom
®
Whole Blood CD4 MicroBeads to be subsequently separated with the autoMACS
®
Pro Separator, the MultiMACS™ Cell24 Separator Plus, or using the Whole Blood Column Kit.

Downstream applications

StraightFrom
®
Whole Blood MicroBeads allow the fast isolation of CD4
+
cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yields of target cells. StraightFrom Whole Blood MicroBeads in combination with the autoMACS
®
Pro Separator allow standardization of the cell separation procedure and safe handling of hazardous samples. The purified CD4
+
cells are well-suited for further flow cytometric, functional, or molecular analysis including lineage-specific chimerism analysis after allogeneic stem cell transplantation
1,2
.

Columns

autoMACS
®
Columns or Whole Blood Columns.
  • Selected references

    1. Meyer, R. G. et al. (2007) Prophylactic transfer of CD8-depleted donor lymphocytes after T-cell-depleted reduced-intensity transplantation. Blood 109: 374-382
    2. Koehl, U. et al. (2003) Quantitative analysis of chimerism after allogeneic stem cell transplantation by PCR amplification of microsatellite markers and capillary electrophoresis with fluorescence detection: the Frankfurt experience. Leukemia 17: 232-236
    3. Adams, S. et al. (2004) Cell lineage-specific chimerism in posthematopoietic stem cell transplant patients. MACS&more 8(2): 16
  • Brochures and posters

Video

How to isolate immune cells directly from blood

Follow this fast and easy protocol for magnetic isolation of immune cells directly from blood or blood products without the need for density gradient centrifugation.

Product options: 1
for 40
mL whole blood
CHF  817.00

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